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CD20 and CD74 labeling by specific fluorescent antibodies. Binding of CD20 and CD74 in mantle cell lymphoma cells was examined by laser-scanning confocal microscopy. Cells were incubated with rhodamine-conjugated rituximab (red) and Alexa Fluor 488-conjugated milatuzumab (green) in the presence of a cross-linking antibody, for 2 hours at 37°C. DRAQ5 was used for nuclear staining (blue). Cross-linked milatuzumab forms large aggregates and colocalizes with cross-linked rituximab on the surface of mantle cell lymphoma cells. See the article by Alinari et al on page 4530.