Recent studies have revealed that the intestinal bacterial microbiome plays an important role in the regulation of hematopoiesis. A correlation between adverse hematologic effects and imbalance of the intestinal microbiome, or dysbiosis, is evident in several human conditions, such as inflammatory bowel disease, obesity, and, critically, in the setting of antibiotic exposure. Here we review the effects of gut dysbiosis on the hematological compartment and our current understanding of the mechanisms through which changes in the bacterial microbiome affect hematopoiesis.

The microbiome influences many biological processes, from early brain development to aging of innate immune cells.1-5  Several recent studies have demonstrated that the bacterial microbiome also plays an important role in normal hematopoiesis.6-9  Human conditions associated with altered intestinal bacterial populations, such as inflammatory bowel syndrome or prolonged antibiotic use, are associated with adverse hematologic effects, including anemia and neutropenia. Understanding the mechanisms by which the microbiome influences normal blood production may help with development of novel treatment modalities to prevent these complications. Here, we discuss the interactions between the microbiome and hematopoiesis, review what is currently known about the mechanisms underlying these connections, and propose a model of signaling between the gut microbiome and bone marrow (BM).

The term microbiome describes the diverse array of microorganisms, including bacteria, viruses, fungi, and archaea, that colonize the human body, forming an ecological system critical to human health.5  Much of what is currently known about the connection between microbiota and hematopoiesis is derived from murine studies. For example, microbiota are entirely lacking in germ-free (GF) mice, and these mice have well-known abnormalities in BM cell populations.6,7,9-11  GF mice have smaller hematopoietic stem and progenitor cell (HSPC) populations, abnormal splenic myeloid counts, and impaired T-cell function compared with their specific-pathogen-free (SPF) counterparts.7,9,10  Similarly, oral antibiotics deplete intestinal bacteria and have suppressive effects on hematopoiesis. Adult SPF mice treated with antibiotics for 1 week or more develop BM suppression, and these effects are largely independent of treatment duration, absorption, and type of antibiotic used.2,7,8,11  Antibiotics have also been shown to disrupt engraftment of HSPCs after transplantation in mice, indicating that the microbiome plays an important role in the posttransplant setting.12  Importantly, biologically relevant concentrations of antibiotics are not toxic to HSPCs under in vitro culture conditions, arguing against a direct antibiotic effect on hematopoiesis.8  Instead, several recent studies, summarized in detail in Table 1, indicate that antibiotics impair normal hematopoiesis by depleting intestinal bacteria. Many of the features of hematopoietic suppression related to microbiota depletion in mice, including timing, are similar to those seen in humans, as we describe below.

Dysbiosis, or imbalance, of the gut microbiome has been linked to suppression of hematopoiesis in humans. Note that the term dysbiosis does not refer to the presence of specific microbial pathogens, which can certainly exert hematologic effects as recently reviewed.13  An imbalance in commensal intestinal bacteria characterizes inflammatory bowel diseases (IBDs), including decreased bacterial diversity, enhanced bacteriophage populations, and outgrowth of pathobionts.14,15  Interestingly, IBD has been independently linked to non-drug-induced aplastic anemia.16,17  A murine model of IBD showed that intestinal inflammation exerts significant stress on HSPCs.18  Nutritional disorders also share a link between dysregulated microbial communities and altered hematological outcomes. For instance, obesity has been associated with both dysbiosis19-21  and hematopoietic abnormalities in humans22,23  and mice.24,25  Malnutrition also is associated with altered intestinal microbial communities,26,27  as well as frequently severe hematological disturbances.28,29  Although these complex conditions in humans have many potential confounding factors, including an altered inflammatory milieu, cellular access to nutrients, and genetic factors, they highlight the potential for microbial dysbiosis and hematological abnormalities to be linked by association or perhaps causally.

A more direct link between microbial dysbiosis and hematopoietic alterations can be observed in patients receiving antibiotics. Antibiotic treatment, which causes gut dysbiosis by eliminating certain classes of bacteria, is widely associated with hematological abnormalities. Cytopenias, including neutropenia, anemia, thrombocytopenia, and pancytopenia, have been reported for a wide range of antibiotics.30-35  For example, a retrospective analysis found that 5% to 15% of patients developed neutropenia (defined as <1000 neutrophils per cubic millimeter) after 10 or more days of β-lactam antibiotic treatment.36  Of the patients that developed neutropenia, 94% recovered neutrophil counts after stopping antibiotic treatment. This finding was corroborated in a review of published clinical case reports that showed patients developed neutropenia when treated with penicillin G.30  These studies suggest that disrupting the gut microbiome through antibiotic use may have a significant impact on hematopoiesis.

Importantly, hematologic abnormalities due to antibiotics are not limited to a single class of antibiotics.30,32-40  Indeed, neutropenia was one of the most common adverse drug-related effects of outpatient parenteral antimicrobial therapy (OPAT) in pediatric patients regardless of the antibiotic agent used.41-44  Confirming this finding, Fernandes et al45  found that leukopenia developed in 6% of pediatric patients on OPAT for a median of 30 days. Of note, the antibiotic trimethoprim-sulfamethoxazole (TMP-SMZ) is widely known to cause neutropenia, but the rate of TMP-SMZ–mediated neutropenia is far lower than that reported for other antibiotics, such as β-lactams. Indeed, the incidence of blood disorders associated with TMP-SMZ (5.6 cases per 100 000 patients) is ∼1000 times lower than that of prolonged antibiotics as described in the OPAT studies mentioned above.46-48  Trimethoprim has been shown to exert an antifolate activity on granulocyte progenitors, an effect that can be reversed by folinic acid administration.49  However, cytopenias related to other antibiotics have not been related to folate deficiency, and their vastly different incidence suggests that the mechanisms of action differ as well.

Similar to the results of recent murine studies, antibiotic treatment can affect the outcome of allogenic hematopoietic stem cell transplant in humans.50-54  Despite the common use of antibiotics following BM transplant, these studies indicate that the reduction in diversity and abundance of intestinal microbiota resulting from antibiotic administration impairs engraftment and increases the risk of leukemic relapse, graft versus host disease, and death following transplant.12,53,55 

In summary, syndromes of dysbiosis are linked to hematologic defects, and 5% to 15% of patients on long-term antibiotic treatment are vulnerable to adverse hematologic complications. Antibiotic courses lasting >2 weeks, when hematologic effects become more common, can be appropriate for many conditions, including osteomyelitis, endocarditis, septic arthritis, and meningitis.43  However, antibiotic-associated neutropenia leaves patients vulnerable to opportunistic and potentially fatal infections, and weekly monitoring for evidence of antibiotic-associated cytopenias is both costly and painful. Stopping or changing antibiotics due to adverse effects adds additional costs and hinders effective treatment, especially for HSCT patients. Thus, understanding the mechanistic basis linking antibiotic use, gut dysbiosis, and hematologic abnormalities is an important clinical priority.

Although long-term antibiotic treatment can clearly affect hematopoiesis, the mechanisms of suppression remain contentious. Several early studies suggested that β-lactam antibiotics directly suppress the differentiation of progenitor cells36,56 ; however, those findings were based on in vitro studies that only showed inhibition at half maximal inhibitory concentration of 100 to 600 μg/mL. These concentrations are much higher than those typically achieved in humans (<50 μg/mL),57  thus calling into question the clinical and biological relevance of these findings. To assess mechanisms of suppression at antibiotic concentrations in the range of those expected in vivo, we cocultured murine BM with the β-lactam antibiotic ceftriaxone (25 μg/mL) or with vancomycin, neomycin, ampicillin, and metronidazole (12.5 μg/mL for vancomycin; 25 μg/mL for the rest) in methylcellulose and showed there was no effect on colony formation.8  These studies indicate that antibiotics at concentrations in the range of those expected in vivo do not antagonize progenitor activity directly.

Conversely, some studies suggested that hematopoietic suppression in patients on long-term antibiotic treatment is caused by indirect, immune-mediated mechanisms.58-60  An antibody-mediated mechanism for vancomycin-associated thrombocytopenia has been established.61  Similarly, detection of antineutrophil and antipenicillin immunoglobulin G antibodies in the sera of neutropenic patients led to the speculation that neutropenia could be a result of antipenicillin antibodies coating penicilloylated neutrophils or opsonization of immune complex coated neutrophils.59,60  Despite these reports, subsequent studies have shown that such effects do not worsen with repeated courses of penicillin, arguing against an antibody-mediated phenomenon.62 

More recently, several groups independently identified changes in the microbiome as a key regulator of normal hematopoiesis (see Table 1).6-9  Suppression of granulocyte and monocyte numbers in GF mice or in antibiotic-treated mice was shown to be rescued by oral provision of MAMPs from the gut microbiota or by recolonization with normal microbiota.6,7  Consistent with these findings, a heat-resistant component of Escherichia coli in serum was found to restore BM myeloid cell populations in GF mice. Furthermore, Iwamura et al9  reported that nucleotide-binding oligomerization domain-containing protein 1 ligand (NOD1L), which is a heat-stable component of the peptidoglycan structure of E coli,63,64  increased systemic levels of HSPC proliferation-stimulating cytokines such as stem cell factor and thrombopoietin to levels found in SPF mice. These growth cytokines are produced in large part by MSCs in the BM niche. In summary, the field is converging on a paradigm in which products of the intestinal microbiota such as NOD1L can enter the bloodstream and travel to the BM, where they promote the production of growth cytokines that support normal hematopoiesis.

Although progress has been made in determining the signals produced by intestinal microbes to trigger normal hematopoiesis, the host cells and receptors by which those signals are detected have not yet been identified. Treatment of MyD88−/−TICAM1−/− GF mice with heat-inactivated serum from SPF mice did not expand the BM myeloid compartment,6  suggesting that MyD88 and TICAM1 are required for appropriate gut-marrow signaling. Our recent study showed that Myd88 single knockout mice had no apparent defect in antibiotic-mediated BM suppression, indicating that MyD88 and TICAM1 may have redundant roles.8  Meanwhile, Stat1−/− mice had BM HSPC and granulocyte counts as low as antibiotic-treated wild-type mice, and treating Stat1−/− mice with antibiotics did not further suppress cell counts, suggesting that STAT1 signaling stimulated by the microbiota is required for normal hematopoiesis.8  Indeed, these data fit into a growing narrative that basal inflammatory signaling is required to maintain normal hematopoiesis.8,65  Recently, Iwamura et al9  determined that NOD1−/− MSCs, in contrast to wild type, are unable to produce HSPC proliferation-stimulating cytokines, indicating that NOD1 signaling through MSCs is an important regulator of hematopoiesis.9  Therefore, it is likely that MyD88/TICAM1, NOD1, and STAT1 are all involved in regulating steady-state hematopoiesis; in fact, it is possible that they all feed into the same pathway. The MyD88-dependent Toll-like receptor (TLR) pathway and NOD1 pathway share similar downstream signaling molecules such as TRAF3, which signals to IRF3 to induce interferon production, and interferons signal via STAT1. Further studies are necessary to confirm the interplay of these proposed signaling pathways (Figure 1) in microbiota-mediated hematopoiesis.

Figure 1.

Proposed model of host signaling cascade in response to microbial signals to promote hematopoiesis. MAMPs activate the TLR pathway, and meso-diaminopimelic acid (DAP) activates the NOD1 pathway in stromal cells. The TLR and NOD1 pathways can cross talk at tumor necrosis factor receptor-associated factor 3 (TRAF3) and induce type I interferon (IFN) production. These type I IFNs can then activate the type I IFN pathway via signal transducer and activator of transcription 1 (STAT1) in HSPCs and activate a gene profile that is necessary to promote hematopoiesis.

Figure 1.

Proposed model of host signaling cascade in response to microbial signals to promote hematopoiesis. MAMPs activate the TLR pathway, and meso-diaminopimelic acid (DAP) activates the NOD1 pathway in stromal cells. The TLR and NOD1 pathways can cross talk at tumor necrosis factor receptor-associated factor 3 (TRAF3) and induce type I interferon (IFN) production. These type I IFNs can then activate the type I IFN pathway via signal transducer and activator of transcription 1 (STAT1) in HSPCs and activate a gene profile that is necessary to promote hematopoiesis.

Close modal

In summary, gut dysbiosis is associated with hematological abnormalities in both humans and mice. Murine studies now show that antibiotic-induced microbiota depletion and BM suppression are due to the absence of heat-stable microbial products that can circulate in the bloodstream and promote hematopoiesis through basal inflammatory signaling. These mechanisms are potentially significant in many patients, including those requiring long-term antibiotic therapy and those recovering from HSCT.

Although studies have begun to uncover the mechanisms of antibiotic-induced hematological adverse effects, many details remain unknown and new questions arise. The range of microbial products that signal to the host to promote normal hematopoiesis and the microbial species from which they derive still need to be elucidated.66-68  Balmer et al6  found that the TLR pathway components MyD88 and TICAM1 are required for steady-state granulopoiesis, but stopped short of determining which microbial product was responsible for activating the TLR signaling. Some microbial metabolites such as short-chain fatty acids have been shown to contribute to the production of hematopoietic precursors in SPF mice,69  but other microbial metabolites, such as from indole or flavonoid metabolism,70,71  have not been studied. In addition, the tissues necessary to transmit signals from gut microbes to hematopoietic progenitors remain unknown. Future research will broaden our understanding of the role microbiota play in regulating hematopoiesis and may identify therapeutic interventions to support healthy hematopoiesis in patients with gut dysbiosis.

The authors would like to thank C. Gillespie for critical reading of the manuscript.

This work was supported by the National Institutes of Health, National Heart, Lung, and Blood Institute grants R01HL136333 and R01HL134880 (K.Y.K. and H.Y.), the Aplastic Anemia and MDS International Foundation Liviya Anderson Award (K.Y.K.), and a March of Dimes Basil O’Connor Starter Scholar Award (K.Y.K.). M.T.B. was supported by the Global Probiotics Council’s Young Investigator Grant for Probiotics Research. H.Y. is supported by National Institutes of Health, National Institute of Diabetes and Digestive and Kidney Diseases grant T32 DK060445.

Contribution: H.Y. wrote the review and made the figures; and M.T.B. and K.Y.K. revised and edited the manuscript.

Conflict-of-interest disclosure: The authors declare no competing financial interests.

Correspondence: Katherine Y. King, Pediatric Infectious Diseases, Baylor College of Medicine, Feigin Center for Pediatric Research, 1102 Bates Ave, Suite 1150, Houston, TX 77030; e-mail: kyk@bcm.edu.

1.
Clarke
TB
,
Davis
KM
,
Lysenko
ES
,
Zhou
AY
,
Yu
Y
,
Weiser
JN
.
Recognition of peptidoglycan from the microbiota by Nod1 enhances systemic innate immunity
.
Nat Med
.
2010
;
16
(
2
):
228
-
231
.
2.
Zhang
D
,
Chen
G
,
Manwani
D
, et al
.
Neutrophil ageing is regulated by the microbiome
.
Nature
.
2015
;
525
(
7570
):
528
-
532
.
3.
Deshmukh
HS
,
Liu
Y
,
Menkiti
OR
, et al
.
The microbiota regulates neutrophil homeostasis and host resistance to Escherichia coli K1 sepsis in neonatal mice
.
Nat Med
.
2014
;
20
(
5
):
524
-
530
.
4.
Dinan
TG
,
Cryan
JF
.
Gut instincts: microbiota as a key regulator of brain development, ageing and neurodegeneration
.
J Physiol
.
2017
;
595
(
2
):
489
-
503
.
5.
Belkaid
Y
,
Harrison
OJ
.
Homeostatic immunity and the microbiota
.
Immunity
.
2017
;
46
(
4
):
562
-
576
.
6.
Balmer
ML
,
Schürch
CM
,
Saito
Y
, et al
.
Microbiota-derived compounds drive steady-state granulopoiesis via MyD88/TICAM signaling
.
J Immunol
.
2014
;
193
(
10
):
5273
-
5283
.
7.
Khosravi
A
,
Yáñez
A
,
Price
JG
, et al
.
Gut microbiota promote hematopoiesis to control bacterial infection
.
Cell Host Microbe
.
2014
;
15
(
3
):
374
-
381
.
8.
Josefsdottir
KS
,
Baldridge
MT
,
Kadmon
CS
,
King
KY
.
Antibiotics impair murine hematopoiesis by depleting the intestinal microbiota
.
Blood
.
2017
;
129
(
6
):
729
-
739
.
9.
Iwamura
C
,
Bouladoux
N
,
Belkaid
Y
,
Sher
A
,
Jankovic
D
.
Sensing of the microbiota by NOD1 in mesenchymal stromal cells regulates murine hematopoiesis
.
Blood
.
2017
;
129
(
2
):
171
-
176
.
10.
Inagaki
H
,
Suzuki
T
,
Nomoto
K
,
Yoshikai
Y
.
Increased susceptibility to primary infection with Listeria monocytogenes in germfree mice may be due to lack of accumulation of L-selectin+ CD44+ T cells in sites of inflammation
.
Infect Immun
.
1996
;
64
(
8
):
3280
-
3287
.
11.
Tada
T
,
Yamamura
S
,
Kuwano
Y
,
Abo
T
.
Level of myelopoiesis in the bone marrow is influenced by intestinal flora
.
Cell Immunol
.
1996
;
173
(
1
):
155
-
161
.
12.
Staffas
A
,
Burgos da Silva
M
,
Slingerland
AE
, et al
.
Nutritional support from the intestinal microbiota improves hematopoietic reconstitution after bone marrow transplantation in mice
.
Cell Host Microbe
.
2018
;
23
(
4
):
447
-
457.e4
.
13.
Manzo
VE
,
Bhatt
AS
.
The human microbiome in hematopoiesis and hematologic disorders
.
Blood
.
2015
;
126
(
3
):
311
-
318
.
14.
Kostic
AD
,
Xavier
RJ
,
Gevers
D
.
The microbiome in inflammatory bowel disease: current status and the future ahead
.
Gastroenterology
.
2014
;
146
(
6
):
1489
-
1499
.
15.
Norman
JM
,
Handley
SA
,
Baldridge
MT
, et al
.
Disease-specific alterations in the enteric virome in inflammatory bowel disease
.
Cell
.
2015
;
160
(
3
):
447
-
460
.
16.
Kishikawa
H
,
Nishida
J
,
Nakano
M
,
Hirano
E
,
Morishita
T
,
Ishii
H
.
Ulcerative colitis associated with aplastic anemia
.
Dig Dis Sci
.
2003
;
48
(
7
):
1376
-
1379
.
17.
Sharma
BC
,
Yachha
SK
,
Mishra
RN
,
Gupta
D
.
Hypoplastic anemia associated with ulcerative colitis in a child
.
J Pediatr Gastroenterol Nutr
.
1996
;
23
(
3
):
326
-
328
.
18.
Griseri
T
,
McKenzie
BS
,
Schiering
C
,
Powrie
F
.
Dysregulated hematopoietic stem and progenitor cell activity promotes interleukin-23-driven chronic intestinal inflammation
.
Immunity
.
2012
;
37
(
6
):
1116
-
1129
.
19.
Le Chatelier
E
,
Nielsen
T
,
Qin
J
, et al
;
MetaHIT consortium
.
Richness of human gut microbiome correlates with metabolic markers
.
Nature
.
2013
;
500
(
7464
):
541
-
546
.
20.
Ley
RE
,
Turnbaugh
PJ
,
Klein
S
,
Gordon
JI
.
Microbial ecology: human gut microbes associated with obesity
.
Nature
.
2006
;
444
(
7122
):
1022
-
1023
.
21.
Kasai
C
,
Sugimoto
K
,
Moritani
I
, et al
.
Comparison of the gut microbiota composition between obese and non-obese individuals in a Japanese population, as analyzed by terminal restriction fragment length polymorphism and next-generation sequencing
.
BMC Gastroenterol
.
2015
;
15
(
1
):
100
.
22.
Herishanu
Y
,
Rogowski
O
,
Polliack
A
,
Marilus
R
.
Leukocytosis in obese individuals: possible link in patients with unexplained persistent neutrophilia
.
Eur J Haematol
.
2006
;
76
(
6
):
516
-
520
.
23.
Bellows
CF
,
Zhang
Y
,
Simmons
PJ
,
Khalsa
AS
,
Kolonin
MG
.
Influence of BMI on level of circulating progenitor cells
.
Obesity (Silver Spring)
.
2011
;
19
(
8
):
1722
-
1726
.
24.
Lee
J-M
,
Govindarajah
V
,
Goddard
B
, et al
.
Obesity alters the long-term fitness of the hematopoietic stem cell compartment through modulation of Gfi1 expression
.
J Exp Med
.
2018
;
215
(
2
):
627
-
644
.
25.
Singer
K
,
DelProposto
J
,
Morris
DL
, et al
.
Diet-induced obesity promotes myelopoiesis in hematopoietic stem cells
.
Mol Metab
.
2014
;
3
(
6
):
664
-
675
.
26.
Blanton
LV
,
Barratt
MJ
,
Charbonneau
MR
,
Ahmed
T
,
Gordon
JI
.
Childhood undernutrition, the gut microbiota, and microbiota-directed therapeutics
.
Science
.
2016
;
352
(
6293
):
1533
.
27.
Smith
MI
,
Yatsunenko
T
,
Manary
MJ
, et al
.
Gut microbiomes of Malawian twin pairs discordant for kwashiorkor
.
Science
.
2013
;
339
(
6119
):
548
-
554
.
28.
el-Nawawy
A
,
Barakat
S
,
Elwalily
T
,
Abdel-Moneim Deghady
A
,
Hussein
M
.
Evaluation of erythropoiesis in protein energy malnutrition
.
East Mediterr Health J
.
2002
;
8
(
2-3
):
281
-
289
.
29.
Santos
EW
,
Oliveira
DC
,
Silva
GB
, et al
.
Hematological alterations in protein malnutrition
.
Nutr Rev
.
2017
;
75
(
11
):
909
-
919
.
30.
Andersohn
F
,
Konzen
C
,
Garbe
E
.
Systematic review: agranulocytosis induced by nonchemotherapy drugs
.
Ann Intern Med
.
2007
;
146
(
9
):
657
-
665
.
31.
Furtek
KJ
,
Kubiak
DW
,
Barra
M
,
Varughese
CA
,
Ashbaugh
CD
,
Koo
S
.
High incidence of neutropenia in patients with prolonged ceftaroline exposure
.
J Antimicrob Chemother
.
2016
;
71
(
7
):
2010
-
2013
.
32.
LaVie
KW
,
Anderson
SW
,
O’Neal
HR
Jr
,
Rice
TW
,
Saavedra
TC
,
O’Neal
CS
.
Neutropenia associated with long-term ceftaroline use
.
Antimicrob Agents Chemother
.
2015
;
60
(
1
):
264
-
269
.
33.
Meissner
HC
,
Townsend
T
,
Wenman
W
, et al
.
Hematologic effects of linezolid in young children
.
Pediatr Infect Dis J
.
2003
;
22
(
9 Suppl
):
S186
-
S192
.
34.
Vinh
DC
,
Rubinstein
E
.
Linezolid: a review of safety and tolerability
.
J Infect
.
2009
;
59
(
suppl 1
):
S59
-
S74
.
35.
Sing
CW
,
Wong
IC
,
Cheung
BM
,
Chan
JC
,
Chu
JK
,
Cheung
CL
.
Incidence and risk estimate of drug-induced agranulocytosis in Hong Kong Chinese. A population-based case-control study
.
Pharmacoepidemiol Drug Saf
.
2017
;
26
(
3
):
248
-
255
.
36.
Neftel
KA
,
Hauser
SP
,
Müller
MR
.
Inhibition of granulopoiesis in vivo and in vitro by beta-lactam antibiotics
.
J Infect Dis
.
1985
;
152
(
1
):
90
-
98
.
37.
Rubinstein
E
,
Isturiz
R
,
Standiford
HC
, et al
.
Worldwide assessment of linezolid’s clinical safety and tolerability: comparator-controlled phase III studies
.
Antimicrob Agents Chemother
.
2003
;
47
(
6
):
1824
-
1831
.
38.
Bayram
N
,
Düzgöl
M
,
Kara
A
,
Özdemir
FM
,
Devrim
İ
.
Linezolid-related adverse effects in clinical practice in children [in Spanish]
.
Arch Argent Pediatr
.
2017
;
115
(
5
):
470
-
475
.
39.
Pai
MP
,
Mercier
RC
,
Koster
SA
.
Epidemiology of vancomycin-induced neutropenia in patients receiving home intravenous infusion therapy
.
Ann Pharmacother
.
2006
;
40
(
2
):
224
-
228
.
40.
Gomez
M
,
Maraqa
N
,
Alvarez
A
,
Rathore
M
.
Complications of outpatient parenteral antibiotic therapy in childhood
.
Pediatr Infect Dis J
.
2001
;
20
(
5
):
541
-
543
.
41.
Maraqa
NF
,
Gomez
MM
,
Rathore
MH
.
Outpatient parenteral antimicrobial therapy in osteoarticular infections in children
.
J Pediatr Orthop
.
2002
;
22
(
4
):
506
-
510
.
42.
Le
J
,
San Agustin
M
,
Hernandez
EA
,
Tran
TT
,
Adler-Shohet
FC
.
Complications associated with outpatient parenteral antibiotic therapy in children
.
Clin Pediatr (Phila)
.
2010
;
49
(
11
):
1038
-
1043
.
43.
Madigan
T
,
Banerjee
R
.
Characteristics and outcomes of outpatient parenteral antimicrobial therapy at an academic children’s hospital
.
Pediatr Infect Dis J
.
2013
;
32
(
4
):
346
-
349
.
44.
Olson
SC
,
Smith
S
,
Weissman
SJ
,
Kronman
MP
.
Adverse events in pediatric patients receiving long-term outpatient antimicrobials
.
J Pediatric Infect Dis Soc
.
2015
;
4
(
2
):
119
-
125
.
45.
Fernandes
P
,
Milliren
C
,
Mahoney-West
HM
,
Schwartz
L
,
Lachenauer
CS
,
Nakamura
MM
.
Safety of outpatient parenteral antimicrobial therapy in children
.
Pediatr Infect Dis J
.
2018
;
37
(
2
):
157
-
163
.
46.
Betts
RF
,
Penn
RL
,
Chapman
SW
, et al
.
Antibiotic use: sulfonamides and trimethoprim-sulfamethoxazole
. In:
Betts
RF
,
Chapman
SW
,
Penn
RL
, et al
, eds. Reese and Betts' A Practical Approach to Infectious Diseases. 5th ed.
Philadelphia, PA
:
Lippincott Williams & Wilkins
;
2003
:
1086
-
1093
.
47.
Keisu
M
,
Wiholm
BE
,
Palmblad
J
.
Trimethoprim-sulphamethoxazole-associated blood dyscrasias. Ten years’ experience of the Swedish spontaneous reporting system
.
J Intern Med
.
1990
;
228
(
4
):
353
-
360
.
48.
Myers
MW
,
Jick
H
.
Hospitalization for serious blood and skin disorders following co-trimoxazole
.
Br J Clin Pharmacol
.
1997
;
43
(
6
):
649
-
651
.
49.
Bjornson
BH
,
McIntyre
AP
,
Harvey
JM
,
Tauber
AI
.
Studies of the effects of trimethoprim and sulfamethoxazole on human granulopoiesis
.
Am J Hematol
.
1986
;
23
(
1
):
1
-
7
.
50.
Peled
JU
,
Devlin
SM
,
Staffas
A
, et al
.
Intestinal microbiota and relapse after hematopoietic-cell transplantation
.
J Clin Oncol
.
2017
;
35
(
15
):
1650
-
1659
.
51.
Weber
D
,
Jenq
RR
,
Peled
JU
, et al
.
Microbiota disruption induced by early use of broad-spectrum antibiotics is an independent risk factor of outcome after allogeneic stem cell transplantation
.
Biol Blood Marrow Transplant
.
2017
;
23
(
5
):
845
-
852
.
52.
Shono
Y
,
van den Brink
MRM
.
Gut microbiota injury in allogeneic haematopoietic stem cell transplantation
.
Nat Rev Cancer
.
2018
;
18
(
5
):
283
-
295
.
53.
Kim
DH
,
Kim
JG
,
Sohn
SK
, et al
.
Clinical impact of early absolute lymphocyte count after allogeneic stem cell transplantation
.
Br J Haematol
.
2004
;
125
(
2
):
217
-
224
.
54.
Peled
JU
,
Jenq
RR
,
Holler
E
,
van den Brink
MR
.
Role of gut flora after bone marrow transplantation
.
Nat Microbiol
.
2016
;
1
(
4
):
16036
.
55.
Goldberg
JD
,
Zheng
J
,
Ratan
R
, et al
.
Early recovery of T-cell function predicts improved survival after T-cell depleted allogeneic transplant
.
Leuk Lymphoma
.
2017
;
58
(
8
):
1859
-
1871
.
56.
Maruyama
T
,
Uchida
K
,
Hara
H
.
Suppressive effect of antibiotics on colony formation from human megakaryocyte progenitors (CFU-M) and granulocyte-macrophage progenitors (CFU-GM)
.
Jpn J Pharmacol
.
1987
;
43
(
4
):
423
-
428
.
57.
Giachetto
G
,
Pirez
MC
,
Nanni
L
, et al
.
Ampicillin and penicillin concentration in serum and pleural fluid of hospitalized children with community-acquired pneumonia
.
Pediatr Infect Dis J
.
2004
;
23
(
7
):
625
-
629
.
58.
Weitzman
SA
,
Stossel
TP
.
Drug-induced immunological neutropenia
.
Lancet
.
1978
;
1
(
8073
):
1068
-
1072
.
59.
Erffmeyer
JE
.
Adverse reactions to penicillin. Part I
.
Ann Allergy
.
1981
;
47
(
4
):
288
-
293
.
60.
Neftel
KA
,
Wälti
M
,
Spengler
H
, et al
.
Neutropenia after penicillins: toxic or immune-mediated? [in German]
.
Klin Wochenschr
.
1981
;
59
(
16
):
877
-
888
.
61.
Von Drygalski
A
,
Curtis
BR
,
Bougie
DW
, et al
.
Vancomycin-induced immune thrombocytopenia
.
N Engl J Med
.
2007
;
356
(
9
):
904
-
910
.
62.
Peng
RR
,
Wu
J
,
Zhao
W
, et al
.
Neutropenia induced by high-dose intravenous benzylpenicillin in treating neurosyphilis: does it really matter?
PLoS Negl Trop Dis
.
2017
;
11
(
3
):
e0005456
.
63.
Hasegawa
M
,
Yang
K
,
Hashimoto
M
, et al
.
Differential release and distribution of Nod1 and Nod2 immunostimulatory molecules among bacterial species and environments
.
J Biol Chem
.
2006
;
281
(
39
):
29054
-
29063
.
64.
Chamaillard
M
,
Hashimoto
M
,
Horie
Y
, et al
.
An essential role for NOD1 in host recognition of bacterial peptidoglycan containing diaminopimelic acid
.
Nat Immunol
.
2003
;
4
(
7
):
702
-
707
.
65.
Fang
J
,
Muto
T
,
Kleppe
M
, et al
.
TRAF6 mediates basal activation of nf-κb necessary for hematopoietic stem cell homeostasis
.
Cell Reports
.
2018
;
22
(
5
):
1250
-
1262
.
66.
Kaiko
GE
,
Ryu
SH
,
Koues
OI
, et al
.
The colonic crypt protects stem cells from microbiota-derived metabolites
.
Cell
.
2016
;
167
(
4
):
1708
-
1720
.
67.
Wang
J
,
Li
F
,
Sun
R
, et al
.
Bacterial colonization dampens influenza-mediated acute lung injury via induction of M2 alveolar macrophages
.
Nat Commun
.
2013
;
4
(
1
):
2106
.
68.
Smith
PM
,
Howitt
MR
,
Panikov
N
, et al
.
The microbial metabolites, short-chain fatty acids, regulate colonic Treg cell homeostasis
.
Science
.
2013
;
341
(
6145
):
569
-
573
.
69.
Trompette
A
,
Gollwitzer
ES
,
Yadava
K
, et al
.
Gut microbiota metabolism of dietary fiber influences allergic airway disease and hematopoiesis
.
Nat Med
.
2014
;
20
(
2
):
159
-
166
.
70.
Sharon
G
,
Garg
N
,
Debelius
J
,
Knight
R
,
Dorrestein
PC
,
Mazmanian
SK
.
Specialized metabolites from the microbiome in health and disease
.
Cell Metab
.
2014
;
20
(
5
):
719
-
730
.
71.
Braune
A
,
Blaut
M
.
Bacterial species involved in the conversion of dietary flavonoids in the human gut
.
Gut Microbes
.
2016
;
7
(
3
):
216
-
234
.
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