Topics:
angiogenesis, blood platelets, bodily secretions, bone marrow

On page 3896 of the 7 April 2011 issue, there is an error in the control panels of Figure 3E. The same tissue was used to represent both the PBS control image for PLT infusion and the rat IgG control image for PLT depletion. Appropriate panels have been used to generate a corrected panel E. The corrected Figure 3 is shown.

Figure 3
Figure 3. Blood vessel density is diminished upon platelet depletion. (A-D) Subcutaneous B16-F10 tumors were implanted in mice undergoing platelet (PLT) depletion or injected with control rat IgG. After 9 days of growth, tumors were excised and sectioned. (A-B) Tumor sections were stained for laminin to visualize blood vessels. Laminin-positive vessels were quantified and represented as mean vessel number per section ± SEM (n = 6). (C-D) Frozen tumor sections were stained for CD31-positive vessels (red) and DAPI (blue). CD31+ blood vessels were quantified and represented as mean percentage of area ± SEM (n = 24). Arrows indicate vessels within the tissues. (E-F) Platelets were depleted or infused in mice directly after hindlimb ischemia surgery. Muscles were collected from mice injected with 3 × 109 platelets (PLT Infusion, Treated) or PBS (PLT Infusion, Control). Muscles were also collected from mice injected with rat anti–mouse GPIbα (PLT Depletion, Treated) or rat IgG (PLT Depletion, Control). Muscles were sectioned 14 days after surgery and immunostained for VWF positive blood vessels. Vessels positive for VWF were counted and represented as mean vessel number per field ± SEM (n = 5). Scale bars represent 50 μm. *P < .05 and ***P < .005 vs control by Student t test.
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Blood vessel density is diminished upon platelet depletion. (A-D) Subcutaneous B16-F10 tumors were implanted in mice undergoing platelet (PLT) depletion or injected with control rat IgG. After 9 days of growth, tumors were excised and sectioned. (A-B) Tumor sections were stained for laminin to visualize blood vessels. Laminin-positive vessels were quantified and represented as mean vessel number per section ± SEM (n = 6). (C-D) Frozen tumor sections were stained for CD31-positive vessels (red) and DAPI (blue). CD31+ blood vessels were quantified and represented as mean percentage of area ± SEM (n = 24). Arrows indicate vessels within the tissues. (E-F) Platelets were depleted or infused in mice directly after hindlimb ischemia surgery. Muscles were collected from mice injected with 3 × 109 platelets (PLT Infusion, Treated) or PBS (PLT Infusion, Control). Muscles were also collected from mice injected with rat anti–mouse GPIbα (PLT Depletion, Treated) or rat IgG (PLT Depletion, Control). Muscles were sectioned 14 days after surgery and immunostained for VWF positive blood vessels. Vessels positive for VWF were counted and represented as mean vessel number per field ± SEM (n = 5). Scale bars represent 50 μm. *P < .05 and ***P < .005 vs control by Student t test.

Figure 3
Figure 3. Blood vessel density is diminished upon platelet depletion. (A-D) Subcutaneous B16-F10 tumors were implanted in mice undergoing platelet (PLT) depletion or injected with control rat IgG. After 9 days of growth, tumors were excised and sectioned. (A-B) Tumor sections were stained for laminin to visualize blood vessels. Laminin-positive vessels were quantified and represented as mean vessel number per section ± SEM (n = 6). (C-D) Frozen tumor sections were stained for CD31-positive vessels (red) and DAPI (blue). CD31+ blood vessels were quantified and represented as mean percentage of area ± SEM (n = 24). Arrows indicate vessels within the tissues. (E-F) Platelets were depleted or infused in mice directly after hindlimb ischemia surgery. Muscles were collected from mice injected with 3 × 109 platelets (PLT Infusion, Treated) or PBS (PLT Infusion, Control). Muscles were also collected from mice injected with rat anti–mouse GPIbα (PLT Depletion, Treated) or rat IgG (PLT Depletion, Control). Muscles were sectioned 14 days after surgery and immunostained for VWF positive blood vessels. Vessels positive for VWF were counted and represented as mean vessel number per field ± SEM (n = 5). Scale bars represent 50 μm. *P < .05 and ***P < .005 vs control by Student t test.
View largeDownload PPT

Blood vessel density is diminished upon platelet depletion. (A-D) Subcutaneous B16-F10 tumors were implanted in mice undergoing platelet (PLT) depletion or injected with control rat IgG. After 9 days of growth, tumors were excised and sectioned. (A-B) Tumor sections were stained for laminin to visualize blood vessels. Laminin-positive vessels were quantified and represented as mean vessel number per section ± SEM (n = 6). (C-D) Frozen tumor sections were stained for CD31-positive vessels (red) and DAPI (blue). CD31+ blood vessels were quantified and represented as mean percentage of area ± SEM (n = 24). Arrows indicate vessels within the tissues. (E-F) Platelets were depleted or infused in mice directly after hindlimb ischemia surgery. Muscles were collected from mice injected with 3 × 109 platelets (PLT Infusion, Treated) or PBS (PLT Infusion, Control). Muscles were also collected from mice injected with rat anti–mouse GPIbα (PLT Depletion, Treated) or rat IgG (PLT Depletion, Control). Muscles were sectioned 14 days after surgery and immunostained for VWF positive blood vessels. Vessels positive for VWF were counted and represented as mean vessel number per field ± SEM (n = 5). Scale bars represent 50 μm. *P < .05 and ***P < .005 vs control by Student t test.

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© 2011 by The American Society of Hematology
2011
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