Topics:
cell death, dimers, dna, genes, glucocorticoid receptors, glucocorticoids, implantable defibrillators, repression, tumor necrosis factor ligand superfamily member 6

On page 620 of the 15 July 2005 issue, the panels in Figure 3C, representing 2 different experimental conditions (with and without CHX), were duplicated. The corrected Figure 3 is shown.

Figure 3
Figure 3. Dex-induced protein synthesis is not required for inhibition of CD95L mRNA expression. (A) Primary murine day-5 T cells were restimulated by anti-CD3 antibody in the presence or absence of 10−7 M Dex for the indicated periods of time. The mRNA expression level of CD95L and β-actin was detected by RT-PCR. (B) Primary murine day-5 T cells were restimulated by anti-CD3 antibody in the presence or absence of 10−7 M Dex for 5 hours. The mRNA expression of CD95L, GILZ, Bcl-2, and β-actin was detected by RT-PCR. (C) Same as in panel B, but restimulation with or without pretreatment for 2 hours with 10 μg/mL CHX. The mRNA expression of CD95L and β-actin was detected by RT-PCR. Results are representative of at least 3 independent experiments.
View largeDownload PPT

Dex-induced protein synthesis is not required for inhibition of CD95L mRNA expression. (A) Primary murine day-5 T cells were restimulated by anti-CD3 antibody in the presence or absence of 10−7 M Dex for the indicated periods of time. The mRNA expression level of CD95L and β-actin was detected by RT-PCR. (B) Primary murine day-5 T cells were restimulated by anti-CD3 antibody in the presence or absence of 10−7 M Dex for 5 hours. The mRNA expression of CD95L, GILZ, Bcl-2, and β-actin was detected by RT-PCR. (C) Same as in panel B, but restimulation with or without pretreatment for 2 hours with 10 μg/mL CHX. The mRNA expression of CD95L and β-actin was detected by RT-PCR. Results are representative of at least 3 independent experiments.

Figure 3
Figure 3. Dex-induced protein synthesis is not required for inhibition of CD95L mRNA expression. (A) Primary murine day-5 T cells were restimulated by anti-CD3 antibody in the presence or absence of 10−7 M Dex for the indicated periods of time. The mRNA expression level of CD95L and β-actin was detected by RT-PCR. (B) Primary murine day-5 T cells were restimulated by anti-CD3 antibody in the presence or absence of 10−7 M Dex for 5 hours. The mRNA expression of CD95L, GILZ, Bcl-2, and β-actin was detected by RT-PCR. (C) Same as in panel B, but restimulation with or without pretreatment for 2 hours with 10 μg/mL CHX. The mRNA expression of CD95L and β-actin was detected by RT-PCR. Results are representative of at least 3 independent experiments.
View largeDownload PPT

Dex-induced protein synthesis is not required for inhibition of CD95L mRNA expression. (A) Primary murine day-5 T cells were restimulated by anti-CD3 antibody in the presence or absence of 10−7 M Dex for the indicated periods of time. The mRNA expression level of CD95L and β-actin was detected by RT-PCR. (B) Primary murine day-5 T cells were restimulated by anti-CD3 antibody in the presence or absence of 10−7 M Dex for 5 hours. The mRNA expression of CD95L, GILZ, Bcl-2, and β-actin was detected by RT-PCR. (C) Same as in panel B, but restimulation with or without pretreatment for 2 hours with 10 μg/mL CHX. The mRNA expression of CD95L and β-actin was detected by RT-PCR. Results are representative of at least 3 independent experiments.

Close modal
© 2011 by The American Society of Hematology
2011
Sign in via your Institution