Topics:
chronic b-cell leukemias, chronic lymphocytic leukemia, disease progression, mice, transgenic

On page 4474 in the November 12, 2009, issue, in Figure 5 panel B, inadvertent errors were made in construction of the day 20 data, with internal duplication of several images. The corrected figure is shown.

Figure 5
Figure 5. CLL cells expand faster in vivo in the presence of constantly elevated levels of CD257. (A) Splenocytes from TCL1×BAFF-Tg mice were labeled with CD3 and CD5 antibodies and CD3−CD5+ were enriched by cell sorting. Sorted leukemic cells (106) were injected into either BAFF-Tg or WT recipients (n = 6 each group) and their expansion was analyzed 30 days later by flow cytometry. (B) Expansion of transferred CD3−CD5+ cells transduced with a luciferase-expressing lentivirus before injection was measured by bioluminescence at the indicated time points. (C) Representative autopsy and flow cytometry of splenocytes from a BAFF-Tg mouse 5 months after adoptive transfer (n = 6). Camera: Nikon COOLPIX 995. (D) Representative flow cytometry of cells obtained from inguinal lymph nodes from WT or BAFF-Tg mice 5 months after adoptive transfer (n = 6 mice each group).
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CLL cells expand faster in vivo in the presence of constantly elevated levels of CD257. (A) Splenocytes from TCL1×BAFF-Tg mice were labeled with CD3 and CD5 antibodies and CD3−CD5+ were enriched by cell sorting. Sorted leukemic cells (106) were injected into either BAFF-Tg or WT recipients (n = 6 each group) and their expansion was analyzed 30 days later by flow cytometry. (B) Expansion of transferred CD3−CD5+ cells transduced with a luciferase-expressing lentivirus before injection was measured by bioluminescence at the indicated time points. (C) Representative autopsy and flow cytometry of splenocytes from a BAFF-Tg mouse 5 months after adoptive transfer (n = 6). Camera: Nikon COOLPIX 995. (D) Representative flow cytometry of cells obtained from inguinal lymph nodes from WT or BAFF-Tg mice 5 months after adoptive transfer (n = 6 mice each group).

Figure 5
Figure 5. CLL cells expand faster in vivo in the presence of constantly elevated levels of CD257. (A) Splenocytes from TCL1×BAFF-Tg mice were labeled with CD3 and CD5 antibodies and CD3−CD5+ were enriched by cell sorting. Sorted leukemic cells (106) were injected into either BAFF-Tg or WT recipients (n = 6 each group) and their expansion was analyzed 30 days later by flow cytometry. (B) Expansion of transferred CD3−CD5+ cells transduced with a luciferase-expressing lentivirus before injection was measured by bioluminescence at the indicated time points. (C) Representative autopsy and flow cytometry of splenocytes from a BAFF-Tg mouse 5 months after adoptive transfer (n = 6). Camera: Nikon COOLPIX 995. (D) Representative flow cytometry of cells obtained from inguinal lymph nodes from WT or BAFF-Tg mice 5 months after adoptive transfer (n = 6 mice each group).
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CLL cells expand faster in vivo in the presence of constantly elevated levels of CD257. (A) Splenocytes from TCL1×BAFF-Tg mice were labeled with CD3 and CD5 antibodies and CD3−CD5+ were enriched by cell sorting. Sorted leukemic cells (106) were injected into either BAFF-Tg or WT recipients (n = 6 each group) and their expansion was analyzed 30 days later by flow cytometry. (B) Expansion of transferred CD3−CD5+ cells transduced with a luciferase-expressing lentivirus before injection was measured by bioluminescence at the indicated time points. (C) Representative autopsy and flow cytometry of splenocytes from a BAFF-Tg mouse 5 months after adoptive transfer (n = 6). Camera: Nikon COOLPIX 995. (D) Representative flow cytometry of cells obtained from inguinal lymph nodes from WT or BAFF-Tg mice 5 months after adoptive transfer (n = 6 mice each group).

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