On page 4474 in the November 12, 2009, issue, in Figure 5 panel B, inadvertent errors were made in construction of the day 20 data, with internal duplication of several images. The corrected figure is shown.
CLL cells expand faster in vivo in the presence of constantly elevated levels of CD257. (A) Splenocytes from TCL1×BAFF-Tg mice were labeled with CD3 and CD5 antibodies and CD3−CD5+ were enriched by cell sorting. Sorted leukemic cells (106) were injected into either BAFF-Tg or WT recipients (n = 6 each group) and their expansion was analyzed 30 days later by flow cytometry. (B) Expansion of transferred CD3−CD5+ cells transduced with a luciferase-expressing lentivirus before injection was measured by bioluminescence at the indicated time points. (C) Representative autopsy and flow cytometry of splenocytes from a BAFF-Tg mouse 5 months after adoptive transfer (n = 6). Camera: Nikon COOLPIX 995. (D) Representative flow cytometry of cells obtained from inguinal lymph nodes from WT or BAFF-Tg mice 5 months after adoptive transfer (n = 6 mice each group).
CLL cells expand faster in vivo in the presence of constantly elevated levels of CD257. (A) Splenocytes from TCL1×BAFF-Tg mice were labeled with CD3 and CD5 antibodies and CD3−CD5+ were enriched by cell sorting. Sorted leukemic cells (106) were injected into either BAFF-Tg or WT recipients (n = 6 each group) and their expansion was analyzed 30 days later by flow cytometry. (B) Expansion of transferred CD3−CD5+ cells transduced with a luciferase-expressing lentivirus before injection was measured by bioluminescence at the indicated time points. (C) Representative autopsy and flow cytometry of splenocytes from a BAFF-Tg mouse 5 months after adoptive transfer (n = 6). Camera: Nikon COOLPIX 995. (D) Representative flow cytometry of cells obtained from inguinal lymph nodes from WT or BAFF-Tg mice 5 months after adoptive transfer (n = 6 mice each group).
