Topics:
dna, double-stranded dna breaks, plasma cells

On pages 3782 through 3789 in the April 16, 2009, issue, there are errors in the text and in the data shown in Figures 1, 5, and 6; in Table 1; and in online-only supplemental Tables 1 and 2. The authors used a cell line they believed to be OPM-1, a multiple myeloma (MM) cell line, for some of their studies. They recently discovered that this cell line is not the original MM OPM-1 cell line, but actually instead represents a line similar or identical to the EBV-transformed line ARH77,1  as characterized by cytogenetics and gene-expression profile.

Table 1

Genes involved in the “DNA damage” functional category, deregulated by Zalypsis in MM1S cells

Probe setGene symbolMM1S FCDescription
212986_s_at TLK2 −2.65 Tousled-like kinase 2 
203725_at GADD45A 2.58 Growth arrest and DNA-damage-inducible, alpha 
222879_s_at POLH 3.34 Polymerase (DNA directed), eta 
203409_at DDB2 3.81 Damage-specific DNA binding protein 2, 48kDa 
205072_s_at XRCC4 −9.32 X-ray repair complementing defective repair in cells 4 
208393_s_at RAD50 −3.93 RAD50 homolog (S cerevisiae) 
208643_s_at XRCC5 −2.50 X-ray repair complementing defective repair in cells 5 
235609_at BRIP1 −2.42 BRCA1-interacting protein C-terminal helicase 1 
239635_at TMEM137 2.52 RNA-binding motif protein 14 
205875_s_at TREX1 2.72 Three prime repair exonuclease 1 
238736_at REV3L 4.18 REV3-like, catalytic subunit of DNA polymerase zeta (yeast) 
Probe setGene symbolMM1S FCDescription
212986_s_at TLK2 −2.65 Tousled-like kinase 2 
203725_at GADD45A 2.58 Growth arrest and DNA-damage-inducible, alpha 
222879_s_at POLH 3.34 Polymerase (DNA directed), eta 
203409_at DDB2 3.81 Damage-specific DNA binding protein 2, 48kDa 
205072_s_at XRCC4 −9.32 X-ray repair complementing defective repair in cells 4 
208393_s_at RAD50 −3.93 RAD50 homolog (S cerevisiae) 
208643_s_at XRCC5 −2.50 X-ray repair complementing defective repair in cells 5 
235609_at BRIP1 −2.42 BRCA1-interacting protein C-terminal helicase 1 
239635_at TMEM137 2.52 RNA-binding motif protein 14 
205875_s_at TREX1 2.72 Three prime repair exonuclease 1 
238736_at REV3L 4.18 REV3-like, catalytic subunit of DNA polymerase zeta (yeast) 
View Large

Accordingly, corrected tables that no longer pool the OPM1 and MM1S analyses, but include only the MM1S data, have been prepared. The corrected Table 1 is shown, and supplemental Tables 1 and 2 have been replaced online. In the text and in the labels of Figure 1, 5, and 6, all occurrences of “OPM-1” should have read “lymphoblastoid cell line.” In the legends of Figures 5 and 6, “OPM-1 cells” should have read “cells from this lymphoblastoid cell line.”

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© 2010 by The American Society of Hematology
2010
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