In the article by Rosati et al entitled “NUP98 is fused to the NSD3 gene in acute myeloid leukemia associated with t(8;11)(p11.2;p15),” which appeared in the May 15, 2002, issue of Blood (Volume 99:3857-3860), the last sentence of the next-to-last paragraph under “Study design” should read, “Using the Expand Long Template PCR System (Roche, Mannheim, Germany) and the appropriate primers, cDNA aliquots were amplified in 10-μL polymerase chain reaction (PCR) as follows: denaturation at 94°C for 5 minutes, followed by 35 amplification cycles (94°C for 50 seconds, 58°C for 20 seconds, and 68°C for 4 minutes), followed by incubation at 68°C for 5 minutes.” Also, the first sentence of the last paragraph under “Study design” should read, “Nested PCR was carried out as follows: denaturation at 94°C for 5 minutes, followed by 35 amplification cycles (94°C for 30 seconds, 58°C for 20 seconds, 68°C for 2 minutes), followed by incubation at 68°C for 5 minutes.”

Sign in via your Institution