Abstract 5010

Abstract Objective:

This study was aimed to investigate the mRNA expression levels of hepatocyte growth factor(HGF), stromal-derived factor-1(SDF-1), Chemokine (C-C motif) ligand 2 (CCL2) and interleukin-8(IL-8) in bone marrow mesenchymal stem cells (MSC) from multiple myeloma (MM) patients.

Methods:

The real time quantitative polymerase chain reaction(RQ-PCR)technique was used to detect the mRAN expression levels of HGF, SDF-1, CCL2 and IL-8 in bone marrow MSC from 20 newly diagnosed MM patients compared with 9 controls.

Results:

The results indicated that the mean mRNA expression level of HGF was up-regulated in MM patients, as compared with control (P < 0.01). However, the mean mRNA expression level of SDF-1 mRNA was down-regulated in MM patients, as compared with control (P < 0.05). There was no significant difference in the mRNA expression levels of CCL2 and IL-8 between MM and control cohorts (P > 0.05).

Conclusion:

The research suggests that multiple chemotaxis-related factors expression of bone marrow microenvironment cellular component are dysrelugulated in MM patients, which might result from the interplay between MM cell and MSC.

Disclosures:

No relevant conflicts of interest to declare.

Topics:
bone marrow, chemotaxis, multiple myeloma, stem cell, mesenchymal, rna, messenger, interleukin-8, monocyte chemoattractant protein-1, stromal cell-derived factor 1, polymerase chain reaction, chemokines

Author notes

*

Asterisk with author names denotes non-ASH members.

© 2010 by The American Society of Hematology
2010
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