In the August 15, 2008, article, the figure legends on the first 4 figures are incorrect. The figures are shown here with their correct legends.
Allelic expression imbalance and mRNA levels of VKORC1 in human liver, left ventricular heart, and B lymphocytes. (A) Allelic expression imbalance (AEI) in liver. Genomic DNA and cDNA were amplified by PCR, and allelic RNA and DNA ratios were measured in samples heterozygous for marker SNP (3730A>G, rs7294) located at 3′-UTR of VKORC1. The A/G ratio for DNA was set to 1, and ratios for cDNA were normalized to that of DNA. Three different conditions were used to measure AEI and the results were combined: E1, cDNA was amplified using primers within exon3; E2 and E3, complete cDNA was amplified. For AEI measurement in E1 and E2, the forward extension primer was used, and for E3, the reverse extension primer. (B) VKORC1 mRNA expression in 65 human liver samples grouped by −1639G>A (or 1173C>T) genotypes. (C,D) AEI in left ventricular heart and B lymphocytes, respectively. Data are means plus or minus SD. *P < .05 versus GG; #P < .05 versus GA, ANOVA with Dunnett posttest.
Allelic expression imbalance and mRNA levels of VKORC1 in human liver, left ventricular heart, and B lymphocytes. (A) Allelic expression imbalance (AEI) in liver. Genomic DNA and cDNA were amplified by PCR, and allelic RNA and DNA ratios were measured in samples heterozygous for marker SNP (3730A>G, rs7294) located at 3′-UTR of VKORC1. The A/G ratio for DNA was set to 1, and ratios for cDNA were normalized to that of DNA. Three different conditions were used to measure AEI and the results were combined: E1, cDNA was amplified using primers within exon3; E2 and E3, complete cDNA was amplified. For AEI measurement in E1 and E2, the forward extension primer was used, and for E3, the reverse extension primer. (B) VKORC1 mRNA expression in 65 human liver samples grouped by −1639G>A (or 1173C>T) genotypes. (C,D) AEI in left ventricular heart and B lymphocytes, respectively. Data are means plus or minus SD. *P < .05 versus GG; #P < .05 versus GA, ANOVA with Dunnett posttest.
Association of VKORC1 SNPs with AEI. Adjusted P values were used to correct for multiple comparisons using Helix Tree software.
Association of VKORC1 SNPs with AEI. Adjusted P values were used to correct for multiple comparisons using Helix Tree software.
The effects of VKORC1 SNPs on RNA splicing and mRNA levels. (A) Relative amounts of 3 splice variants of VKORC1 in 65 liver samples. The splice variants were measured by PCR using fluorescently labeled primers. (B) Splice variants of VKORC1 in 4 cell lines after transfection with the entire transcribed region of VKORC1. Haplotype A (1173 T, 1542 C, 2255 T) or haplotype B (1173 C, 1542 G, 2255 C) in pcDNA vector was transfected separately into different cell lines, and VKORC1 splice variants measured. (C) Allelic RNA expression of VKORC1 in 4 cell lines after cotransfection with VKORC1 haplotype A and B, using 3730A>G as marker. Haplotype B contains the 3730 G allele, whereas haplotype A contains the 3730 A allele. Data are means plus or minus SD.
The effects of VKORC1 SNPs on RNA splicing and mRNA levels. (A) Relative amounts of 3 splice variants of VKORC1 in 65 liver samples. The splice variants were measured by PCR using fluorescently labeled primers. (B) Splice variants of VKORC1 in 4 cell lines after transfection with the entire transcribed region of VKORC1. Haplotype A (1173 T, 1542 C, 2255 T) or haplotype B (1173 C, 1542 G, 2255 C) in pcDNA vector was transfected separately into different cell lines, and VKORC1 splice variants measured. (C) Allelic RNA expression of VKORC1 in 4 cell lines after cotransfection with VKORC1 haplotype A and B, using 3730A>G as marker. Haplotype B contains the 3730 G allele, whereas haplotype A contains the 3730 A allele. Data are means plus or minus SD.
The effects of promoter SNP on VKORC1 mRNA expression. (A) Transcriptional activity of promoter fragments containing −1639 G or −1639 A, measured by luciferase reporter gene assays in 4 different cell lines. (B) Effects of 5′ upstream fragments F1 and F2 on transcription activity of proximate promoter fragment F3. (C) Allelic mRNA expression of VKORC1 in HepG2 cells after cotransfection with VKORC1 gene constructs. The constructs combine −1639 G or −1639 A alleles with either haplotype A or B of the transcribed region. Data are means plus or minus SD.
The effects of promoter SNP on VKORC1 mRNA expression. (A) Transcriptional activity of promoter fragments containing −1639 G or −1639 A, measured by luciferase reporter gene assays in 4 different cell lines. (B) Effects of 5′ upstream fragments F1 and F2 on transcription activity of proximate promoter fragment F3. (C) Allelic mRNA expression of VKORC1 in HepG2 cells after cotransfection with VKORC1 gene constructs. The constructs combine −1639 G or −1639 A alleles with either haplotype A or B of the transcribed region. Data are means plus or minus SD.
This feature is available to Subscribers Only
Sign In or Create an Account Close Modal