On page 3325 in the 5 April 2012 issue, there is an error in Figure 3D. The incorrect immunoblot for Lon was shown. This error has been corrected in the figure shown below. The error does not affect any other data or conclusions of the paper.
CDDO directly interacts with purified Lon and forms a complex with Lon in cultured cells. (A) CDDObiotin (2.5 μM) was reacted with purified Lon (2 μγ) in PBS containing 1% NP40 for 90 minutes at room temperature, after which CDDObiotin was pulled down with SA-sepharose (SA-seph). (B) Granta cells were treated with or without CDDO or CDDObiotin for 1 hour at 37°C, followed by extraction of cellular proteins and incubation with SA-seph as indicated for 90 minutes at 4°C; (C-D) LS174T or Granta cells extracts (20 μg), respectively, were reacted with or without CDDO or CDDObiotin for 90 minutes at 4°C, followed by SA-seph as in panel B. Pull-down reactions were immunoblotted with antibodies to Lon, ClpP, or BiP. Untreated mock samples contained input but without CDDObiotin and SA-seph.
CDDO directly interacts with purified Lon and forms a complex with Lon in cultured cells. (A) CDDObiotin (2.5 μM) was reacted with purified Lon (2 μγ) in PBS containing 1% NP40 for 90 minutes at room temperature, after which CDDObiotin was pulled down with SA-sepharose (SA-seph). (B) Granta cells were treated with or without CDDO or CDDObiotin for 1 hour at 37°C, followed by extraction of cellular proteins and incubation with SA-seph as indicated for 90 minutes at 4°C; (C-D) LS174T or Granta cells extracts (20 μg), respectively, were reacted with or without CDDO or CDDObiotin for 90 minutes at 4°C, followed by SA-seph as in panel B. Pull-down reactions were immunoblotted with antibodies to Lon, ClpP, or BiP. Untreated mock samples contained input but without CDDObiotin and SA-seph.
