On page 5118 in the 15 June 2007 issue, there are errors in Figure 6E. The images labeled as Medium and Gossypin were erroneously duplicated in the original figure. The appropriate Medium and Gossypin independent experimental images are now shown in the corrected Figure 6E.
Gossypin inhibits TNF-induced invasion and RANKL-induced osteoclastogenesis. (A) Gossypin suppressed TNF-induced invasion. H1299 cells were seeded in the top of a Matrigel invasion chamber overnight in the absence of serum, preincubated with 50 μM gossypin for 2 hours, treated with 1 nmol/LTNF for 24 hours in the presence of 1% serum, and then subjected to an invasion assay, as described in “Materials and methods.” (B and C) Gossypin inhibited RANKL-induced osteoclastogenesis. RAW 264.7 cells were incubated alone or with RANKL (5 nmol/L), with or without 50 μM gossypin, for 5 days and stained for TRAP expression. (B,D) Multinucleated (three nuclei) osteoclasts were counted. (C) TRAP-positive cells were photographed (10× 0.25 objective, TMS, Nikon, Tokyo, Japan; Coolpix 950 color camera, Nikon, Lewisville, TX). Original magnification, ×100. (D-E) Gossypin inhibited RANKL-induced osteoclastogenesis. Mouse (C57BL/6J) primary bone marrow cells were incubated alone or with RANKL (5 nmol/L), with or without 50 μM gossypin, for 3 days and stained for TRAP expression. (D) Multinucleated (three nuclei) osteoclasts were counted. (E) TRAP-positive cells were photographed. Original magnification, ×100.
Gossypin inhibits TNF-induced invasion and RANKL-induced osteoclastogenesis. (A) Gossypin suppressed TNF-induced invasion. H1299 cells were seeded in the top of a Matrigel invasion chamber overnight in the absence of serum, preincubated with 50 μM gossypin for 2 hours, treated with 1 nmol/LTNF for 24 hours in the presence of 1% serum, and then subjected to an invasion assay, as described in “Materials and methods.” (B and C) Gossypin inhibited RANKL-induced osteoclastogenesis. RAW 264.7 cells were incubated alone or with RANKL (5 nmol/L), with or without 50 μM gossypin, for 5 days and stained for TRAP expression. (B,D) Multinucleated (three nuclei) osteoclasts were counted. (C) TRAP-positive cells were photographed (10× 0.25 objective, TMS, Nikon, Tokyo, Japan; Coolpix 950 color camera, Nikon, Lewisville, TX). Original magnification, ×100. (D-E) Gossypin inhibited RANKL-induced osteoclastogenesis. Mouse (C57BL/6J) primary bone marrow cells were incubated alone or with RANKL (5 nmol/L), with or without 50 μM gossypin, for 3 days and stained for TRAP expression. (D) Multinucleated (three nuclei) osteoclasts were counted. (E) TRAP-positive cells were photographed. Original magnification, ×100.
