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Laser scanning confocal microscopy of fibrin clots with recombinant M1 protein (0-60 μg/mL). Fibrinogen (Alexa Fluor 594) in human plasma was clotted, imaged as 20-μm Z-stacks (58 slices), and reconstructed in Imaris to generate 3-dimensional isosurfaces. The color scale reflects fibrin intensity. Scale bar, 20 μm. In plasma-only controls, a continuous fibrin film (arrow) overlays a distinct fibrin fiber network. With increasing M1, the film becomes progressively porous and lower intensity; at 60 μg/mL, it is absent or indistinguishable from the underlying network. See the article by Cherrington et al.