Affinity chromatography of human cryosupernatants on anti-human factor VIII-Sepharose yielded a plasma devoid of detectable factor VIIIC, VIIIR:Ag, and VIIIR:WF activities. This plasma was indistinguishable from severe congenital hemophilic plasma when used as substrate in factor VIII coagulant assays.

Topics:
factor viii, plasma, chromatography, affinity, coagulants, sepharose
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Copyright © 1980 by The American Society of Hematology
1980
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