An improved method for doing DFP32 neutrophil survival studies is described that allows quantitative measurement of total circulating neutrophil-associated radioactivity. DFP32 labeled neutrophils are quantitatively trapped on a nylon fiber column and washed free of contaminating radioactivity. The neutrophil-associated radioactivity is then counted in a liquid scintillation counter. Data so obtained can then be used to construct both a true neutrophil survival curve and a curve for the fall-off of neutrophil specific activity. Leukokinetic data on hematologically normal individuals compare favorably with the values for normal persons that Athens and his collaborators have published.

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© 1970 by American Society of Hematology, Inc.
1970
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