The Use of Homogenized Human Leukocytes for the Preparation of Anti-Lymphocyte Serum

This is the first report of the preparation of a potent antileukocyte serum produced by immunization with homogenates of human leukocytes. High titers of antibody activity were found against nuclei and a number of other intracellular materials, including several leukocyte enzymes. This antiserum, prepared in rabbits, also had marked in vitro lymphocyte stimulating activity that compared favorable with the activity of antilymphocyte serum prepared with intact cells. Cytotoxicity was enhanced in the presence of complement.