In order to investigate the cellular distribution of catalase in normal, hypocatalasic and acatalasic red blood cells, the fluorescent antibody labelling technic was employed. Sensitive anticatalase sera were produced in rabbits by immunization with purified catalase extracted from human erythrocytes. Specificity against human erythrocyte catalase was confirmed by Ouchterlony’s double diffusion method.

The distribution of catalase is fairly homogeneous in normal and hypocatalasic red cells, but in acatalasic cells fluorescence due to the presence of catalase was not observed.

By this method the amount of catalase in hypocatalasic red cells was judged to be between that of normal and acatalasic red cells.

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© 1969 by American Society of Hematology, Inc.
1969
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