Inhomogeneities in Alkali-Resistant Hemoglobin. Demonstration of Zone Electrophoretic Differences Using a Cationic Detergent Electrolyte

Trimethyloctadecyl ammonium chloride, a cationic active detergent electrolyte, when added to starch gels before electrophoresis, caused selected alteration in the migration rates of alkali-resistant hemoglobin. Other red blood cell pigments were not similarly affected. Electrophoretic inhomogeneity of cord blood and thalassemia hemoglobin was noted. New components of cord blood hemoglobin were revealed by the method. A new component in the hemoglobin of cases of severe thalassemia was demonstrated.