On page 835, the western blot at the bottom of Figure 5B is a duplicate copy of the western blot at the bottom of Figure 5C. In the description of Figure 5B in the figure legend, “Lane 8: expression control for FBXW7, lysate from FBXW7 transfected cells, no-CoIP” should read “Lane 8: lysate from untransfected cells, no-CoIP.” The authors have inserted the correct blot at the bottom of Figure 5B, and the corrected Figure 5 and legend are shown below. This error does not affect the results or the conclusions of the study.
Effect of patient-specific mutations in FBXW7 on NICD binding. (A-C) Coimmunoprecipitation (CoIP) of NOTCH1-IC (NICD) with wild-type or mutant FBXW7 constructs. HEK293 cells were cotransfected with NICD (which corresponds to the human NOTCH1 intracellular domain, aa 1761-2555) and the indicated Flag-tagged FBXW7α constructs. Expression of NICD (middle panel) and Flag-FBXW7 proteins (bottom panel) was verified by western blotting. CoIPs were performed 24 hours after transfection. ∗The heavy chain of anti-Flag antibody used for IP. Localization of the corresponding mutations is shown schematically in the topmost panel. (B) Lane 8: lysate from untransfected cells, no-CoIP. Control CoIPs for testing unspecific binding to the Flag column are shown in supplemental Figure 2. hs-NICD, Homo sapiens-NICD; IP, immunoprecipitation; WB, western blot.
Effect of patient-specific mutations in FBXW7 on NICD binding. (A-C) Coimmunoprecipitation (CoIP) of NOTCH1-IC (NICD) with wild-type or mutant FBXW7 constructs. HEK293 cells were cotransfected with NICD (which corresponds to the human NOTCH1 intracellular domain, aa 1761-2555) and the indicated Flag-tagged FBXW7α constructs. Expression of NICD (middle panel) and Flag-FBXW7 proteins (bottom panel) was verified by western blotting. CoIPs were performed 24 hours after transfection. ∗The heavy chain of anti-Flag antibody used for IP. Localization of the corresponding mutations is shown schematically in the topmost panel. (B) Lane 8: lysate from untransfected cells, no-CoIP. Control CoIPs for testing unspecific binding to the Flag column are shown in supplemental Figure 2. hs-NICD, Homo sapiens-NICD; IP, immunoprecipitation; WB, western blot.
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