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On page 834, Figure 6F has an error that occurred during preparation of the figure. The 2 images that show the XBP-1U and XBP-1s bands in LP1 cells after treatment with bortezomib (Btz) and tunicamycin (Tun) are actually the same one, namely, the image of Btz-treated cells. The corrected Figure 6F is shown below. The authors apologize for the error.
Figure 6.
The role of XBP1 in UPR-mediated Mcl-1 expression. (A-F) RT-PCR analysis of XBP1 splicing in different MM cell lines. The MM cell lines OPM2, MMS1, U266, 5T33vt, RPMI-8226, and LP1 were treated with bortezomib and tunicamycin for 24 hours at varying doses. RNA isolation and RT-PCR were performed as described in “Methods.” In the human MM cell lines OPM2, MMS1, U266, RPMI-8226, and LP1, the 210- and 184-bp DNA fragments correspond to unspliced and spliced human XBP1 mRNAs, respectively. In 5T33vt cells, the 343- and 327-bp DNA fragments correspond to unspliced and spliced mouse Xbp1 mRNAs, respectively.
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The role of XBP1 in UPR-mediated Mcl-1 expression. (A-F) RT-PCR analysis of XBP1 splicing in different MM cell lines. The MM cell lines OPM2, MMS1, U266, 5T33vt, RPMI-8226, and LP1 were treated with bortezomib and tunicamycin for 24 hours at varying doses. RNA isolation and RT-PCR were performed as described in “Methods.” In the human MM cell lines OPM2, MMS1, U266, RPMI-8226, and LP1, the 210- and 184-bp DNA fragments correspond to unspliced and spliced human XBP1 mRNAs, respectively. In 5T33vt cells, the 343- and 327-bp DNA fragments correspond to unspliced and spliced mouse Xbp1 mRNAs, respectively.

Figure 6.
The role of XBP1 in UPR-mediated Mcl-1 expression. (A-F) RT-PCR analysis of XBP1 splicing in different MM cell lines. The MM cell lines OPM2, MMS1, U266, 5T33vt, RPMI-8226, and LP1 were treated with bortezomib and tunicamycin for 24 hours at varying doses. RNA isolation and RT-PCR were performed as described in “Methods.” In the human MM cell lines OPM2, MMS1, U266, RPMI-8226, and LP1, the 210- and 184-bp DNA fragments correspond to unspliced and spliced human XBP1 mRNAs, respectively. In 5T33vt cells, the 343- and 327-bp DNA fragments correspond to unspliced and spliced mouse Xbp1 mRNAs, respectively.
View largeDownload PPT

The role of XBP1 in UPR-mediated Mcl-1 expression. (A-F) RT-PCR analysis of XBP1 splicing in different MM cell lines. The MM cell lines OPM2, MMS1, U266, 5T33vt, RPMI-8226, and LP1 were treated with bortezomib and tunicamycin for 24 hours at varying doses. RNA isolation and RT-PCR were performed as described in “Methods.” In the human MM cell lines OPM2, MMS1, U266, RPMI-8226, and LP1, the 210- and 184-bp DNA fragments correspond to unspliced and spliced human XBP1 mRNAs, respectively. In 5T33vt cells, the 343- and 327-bp DNA fragments correspond to unspliced and spliced mouse Xbp1 mRNAs, respectively.

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