Abstract 4832

To explore the expression level of insulin-like growth factor-1 receptor (IGF-IR) in malignant clone cells of myelodysplastic syndromes (MDS).

Method

Fluorescence in situ hybridization (FISH) and immunochemistry (APAAP, alkaline phosphatase anti-alkaline phosphatase) were used together to detect the expression of IGF-IR in the bone marrow cells of 26 MDS patients with known abnormal karyotypes.

Result

The average IGF-IR expression level on the surface of clone cells from the 26 MDS cases was markedly elevated compared to the corresponding level in normal cells (78.2±13.7% vs 14.1±14.0%,P <0.0001). The percentages of malignant clone cells in all 26 MDS cases were significantly correlated with the respective percentages of IGF-IR positive nucleated cells (r = 0.909; P <0.0001). No significant difference in the IGF-IR expression level on the clone cells were observed either between high- and low-risk MDS patients or among MDS patients with different abnormal karyotypes.

Conclusion

IGF-IR might be taken as a marker of clone cells in MDS because of its propensity to cause malignant proliferation.

Disclosures

No relevant conflicts of interest to declare.

Topics:
bone marrow, myelodysplastic syndrome, karyotype determination procedure, alkaline phosphatase, insulin-like growth factor i, phosphoric monoester hydrolases, fluorescent in situ hybridization, immunochemistry, clone cells, bone marrow cells

Author notes

*

Asterisk with author names denotes non-ASH members.

© 2009 by The American Society of Hematology
2009
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