Evaluation of Stem Cell Concentrate Prepared From Bone Marrow Aspirate Using the Res-Q^TM system.

The Res-Q System is a point of care device intended for the preparation of cell concentrates from bone marrow by centrifugation. Stem cell concentrates from bone marrow aspirate (BMA) were prepared in less than 15 minutes. The bone marrow concentrate was collected in the device funnel and extracted in a harvesting syringe. The cellular components of samples were analyzed pre- and post processing.

For this study 4 anticoagulated bone marrows were processed. All samples were evaluated for cell recovery, hematocrit, total nucleated cells (TNC), mononuclear cells (MNC), CD34+ cells, cell viability and colony forming units before and after Res-Q60 processing. The Res Q processed post samples were cultured and cells characterized by flow cytometry using cell surface markers.

The Res-Q60 process achieved MNC fraction volumes of 6.7 ± 0.1 ml with a final average hematocrit of 17± 0.6%. The post-processing recovery of CD34+ cells was 90 ±12.7%, of MNC was 85 ± 4% and of TNC was 64 ± 13%. No change in cell viability was observed using the Res-Q60 device. Res-Q derived cell concentrate cultured in mesenchymal stem cells basal media followed by immunophenotyping measured by flow cytometry were ≥ 95% positive for CD73, CD90 and CD105. The same cells lack (≤ 5%) expression of CD31 and CD34.

In conclusion, Res-Q60 system provides a new simple method for preparation of cell concentrates from bone marrow at the point of care.

Kumar:Thermogenesis Corp.: Employment. Chapman:Thermogenesis Corp.: Employment.