Objective:To investigate the bone marrow function of stem cells in patients with systemic lupus erythematosus (SLE), and to explore the pathogenesis of cytopenia in patients with SLE.

Methods:Sixteen patients with SLE and 10 healthy controls were studied for in vitro cultures of CFU-E, BFU-E and CFU-GM from bone marrow mononuclear cells.

Results:49.43±34.65 of CFU-E colonies per 105 BMMCs, 49.2±39.61 of CFU-GM colonies per 105 BMMNCs and 3.01±4.54 of BFU-E colonies per 105 BMMCs were seen in the group of SLE patients with normal blood count. 143.33±152.8 of CFU-E colonies per 105 BMMCs, 122.2±169.87 of CFU-GM colonies per 105 BMMCs, and 2.76±3.28 of BFU-E colonies per 105 BMMCs were seen in the group of SLE with hemocytopenia. 66.3±12.95 of CFU-E colonies per 105 BMMCs, 36.7±11.95 of CFU-GM colonies per 105 BMMCs, and 36±11.66 of BFU-E colonies per 105 BMMCs were seen in the group of normal controls.The quantity of BFU-E colonies per 105 BMMCs in SLE patients were significant lower than that in normal controls. There were no significant differences of the quantities of CFU-GM and CFU-E colonies between the SLE patients and normal controls.

Conclusions: 1. There were no significant differences of CFU-GM and CFU-E colonies between the SLE patients and normal controls, that suggested that SLE patients have normal proliferate function of bone marrow hematopoietic stem cells.2 There were significant lower BFU-E colonies per 105 BMMCs in SLE patients than that in normal controls.

Topics:
bone marrow, hematopoietic stem cells, systemic lupus erythematosus, cytopenia, blood cell count, erythroid progenitor cells, mononuclear cells, stem cells

Disclosure: No relevant conflicts of interest to declare.

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2006, The American Society of Hematology
2006
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