An attempt was made to establish an SCID-hu murine model of long-term human hematopoiesis by coimplantation of a bone fragment and bone marrow (BM) cells from an adult human. The SCID-hu mice were treated with a cytokine mixture (recombinant human stem cell factor, interleukin-3, granulocyte/macrophage colony-stimulating factor, and granulocyte colony-stimulating factor) for 4 months and were then maintained for further 8 months under cytokine-free conditions. In the peripheral blood, spleen, and implanted bone fragments in the SCID-hu mice that had received both a bone fragment and BM cells, human CD59+ cells were detected 1 year after transplantation; however, they were not detectable in SCID-hu mice that had received either a bone fragment or BM cells only. Thus, implantation of both a bone fragment and BM cells appears to provide a model of long-term adult human hematopoiesis in SCID mice.
ARTICLES|
July 15, 1995
Long-term human hematopoiesis in SCID-hu mice bearing transplanted fragments of adult bone and bone marrow cells
Y Heike,
Y Heike
Pharmacology Division, National Cancer Center Research Institute, Tokyo, Japan.
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T Ohira,
T Ohira
Pharmacology Division, National Cancer Center Research Institute, Tokyo, Japan.
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M Takahashi,
M Takahashi
Pharmacology Division, National Cancer Center Research Institute, Tokyo, Japan.
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N Saijo
N Saijo
Pharmacology Division, National Cancer Center Research Institute, Tokyo, Japan.
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Blood (1995) 86 (2): 524–530.
Citation
Y Heike, T Ohira, M Takahashi, N Saijo; Long-term human hematopoiesis in SCID-hu mice bearing transplanted fragments of adult bone and bone marrow cells. Blood 1995; 86 (2): 524–530. doi: https://doi.org/10.1182/blood.V86.2.524.bloodjournal862524
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