Abstract
Both cytotoxic and agglutinating antibodies developed predictably in response to repeated intradermal injections of small amounts of leukocytes. In 3 of 5 such immunizations, the resulting antisera were monospecific and the donor-recipient matches revealed few antigenic differences. Restimulation caused a prompt appearance of antibody in high titer. Attempts to characterize a single serum sample in terms of its leukoagglutinating and cytotoxic activity may not be valid unless the serum used is known to contain comparable levels of antibody activity.
Further advances in leukocyte typing for organ transplantation should be facilitated by the availability of monospecific typing sera. Sera of this nature can be produced by immunization of recipients with closely matched donors.
