Brief Report: Rapid Determination of Vitamin B_l2-Binding ^α and ^β 3 Globulin in Serum

A procedure has been described for the rapid and separate measurement of B₁₂-binding α- and β-globulins using the batch technic. The method involves saturating B₁₂-binders with Co⁵⁷B₁₂, removing excess radioactive B₁₂ with a hemoglobin-coated charcoal pellet, and then separating the B₁₂ binders using a pellet of DEAE cellulose, from which only the β-globulins are eluted in 0.06 M phosphate buffer at pH 6.35.