Various methods of freezing have been evaluated for the preservation of the radiation protection effect in fetal mouse liver. The best results were obtained with tissue frozen slowly in glycerol to -80 C., stored at -50 C. or lower, and thawed rapidly. Other variables such as the size of the tissue particles, the amount of serum in the freezing mixture, and the method of dilution did not influence the results.

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© 1961 by American Society of Hematology, Inc.
1961
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