Both myeloproliferative neoplasms (MPNs) and coronavirus disease 2019 (COVID-19) are characterized by an intrinsic thrombotic risk. Little is known about the incidence and the outcome of thrombotic events in patients with MPN infected by severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), but common mechanisms of coagulation activation, typical of both disorders, suggest that these patients can be at particularly high risk. To define the best thromboprophylaxis and treatment regimens in both MPN and COVID-19, individual- and disease-specific thrombotic risk factors, bleeding risk, and concomitant specific treatments need to be considered. In this case-based review, an individualized approach is presented in a case of SARS-CoV-2 infection occurring in a man with polycythemia vera (PV). A primary anticoagulant thromboprophylaxis strategy and adjustment of his PV treatment were implemented. However, during the hospital stay, he experienced pulmonary embolism and therapeutic anticoagulation had to be set. Then his condition improved, and discharge was planned. Postdischarge decisions had to be made about the type and duration of venous thromboembolism treatment as well as the management of PV-specific drugs. The steps of our decisions and recommendations are presented.

Learning Objectives

  • To learn the current risk-adapted approaches to prevent VTE in patients with MPN, COVID-19, or both

  • To identify the appropriate strategies for initial, long-term, and extended treatment of VTE and for MPN-specific therapy management in patients with MPN, thrombosis, and COVID-19

BCR/ABL-negative myeloproliferative neoplasms (MPNs), including polycythemia vera (PV), essential thrombocythemia (ET), myelofibrosis (MF), and prefibrotic MF (pre-MF), carry a high risk of thrombosis, which affects morbidity and mortality. The disease management, particularly in PV and ET, remains highly dependent on the patient's thrombotic risk.1  Recently, the coronavirus disease 2019 (COVID-19) pandemic caused by severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) spread all over the world, causing millions of deaths. The severe form of COVID-19 is characterized by interstitial pneumonia, multiorgan dysfunction, and a high thrombotic incidence, especially venous thromboembolism (VTE).2 

MPN and COVID-19 share common pathogenic pathways of hypercoagulability and thrombosis (Figure 1). In MPN, platelets, erythrocytes, and leukocytes stemming from the clonal proliferation of hematopoietic progenitor cells are constitutively activated.3  Furthermore, inflammatory mechanisms are highly involved and contribute to the overexpression of adhesion molecules by blood cells and endothelial cells (ECs), favoring cellular interactions and thrombosis. Moreover, JAK2 V617F has been detected in mature ECs in MPN, showing that JAK2-mutant ECs are prothrombotic by overexpressing P-selectin.4  In COVID-19, thromboinflammation, triggered by the viral infection, is a very important thrombogenic mechanism. An excessive immune response, named the “cytokine storm,” together with hypoxia and endothelial damage, is involved5,6  and precipitates the occurrence of thrombosis.

Figure 1.

Specific and common mechanisms of hypercoagulability and thromboinflammation in MPN and COVID-19. Mechanisms of hypercoagulability in MPN and COVID-19 share common pathways. Major differences among the 2 diseases rely on the type of trigger of clotting activation, which in COVID-19 is due to the action of acute infection by SARS-CoV-2 on the prothrombotic characteristics of various hemostatic compartments. A maladaptive hyperactivation of innate immune systems in COVID-19 is also responsible for the activation of the complement cascade and endothelial dysfunction. Differently, in MPN, the systemic hypercoagulability results from the exposure to a long-term (chronic) subinflammatory condition, caused by the abnormal and clonal proliferation of JAK2-mutated myeloid cells.

Figure 1.

Specific and common mechanisms of hypercoagulability and thromboinflammation in MPN and COVID-19. Mechanisms of hypercoagulability in MPN and COVID-19 share common pathways. Major differences among the 2 diseases rely on the type of trigger of clotting activation, which in COVID-19 is due to the action of acute infection by SARS-CoV-2 on the prothrombotic characteristics of various hemostatic compartments. A maladaptive hyperactivation of innate immune systems in COVID-19 is also responsible for the activation of the complement cascade and endothelial dysfunction. Differently, in MPN, the systemic hypercoagulability results from the exposure to a long-term (chronic) subinflammatory condition, caused by the abnormal and clonal proliferation of JAK2-mutated myeloid cells.

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Data on thrombosis in patients with both MPN and COVID-19 are limited, but it is likely that patients with MPN who become infected are more susceptible to develop thrombotic complications.7  In this case-based review, my approach to prevent and treat VTE in a patient with PV and COVID-19 is presented.

CLINICAL CASE

March 2020: a 71-year-old male patient presents to the emergency room with fever, generalized weakness, cough, lethargy, and dyspnea. His medical history was remarkable for PV from 2016, currently being treated with oral aspirin 100 mg daily and ruxolitinib 10 mg twice daily due to intolerance to hydroxyurea (HU), with good disease control; he had stopped requiring therapeutic phlebotomy for 2 years. He also had diabetes, hypertension, and history of transient ischemic attack. His body mass index was 27.2 kg/m2.

On admission to the emergency room, he was screened for SARS-CoV-2 by real-time reverse transcriptase–polymerase chain reaction assay on nasal swab, which proved to be positive. Blood pressure was 130/70 mm Hg, heart rate was 123 beats/min, and body temperature was 38.7°C. The patient's respiratory rate was 18 breaths/min and oxygen saturation was 75% on room oxygen.

A chest x-ray showed large bilateral multiple lung shadows. The laboratory findings included hemoglobin, 145 g/L; white blood cell, 13 × 109/L; and platelet count, 328 × 109/L.

Due to the severity of SARS-CoV-2 infection, he was hospitalized and commenced on oral hydroxychloroquine for 5 days and oral lopinavir/ritonavir, plus large-spectrum antibiotics, as this was the standard approach in March 2020.

We debated on whether and which VTE anticoagulant thromboprophylaxis to adopt and how to manage PV-specific treatment (aspirin and ruxolitinib).

MPN

Patients with MPN have a 3- and 10-fold higher risk of arterial and venous thrombosis, respectively, compared with controls.8  Arterial thromboses account for approximately two-thirds of total thrombotic events.9  Venous thrombosis represents the remaining one-third, including deep venous thrombosis (DVT) of the legs and/or pulmonary embolism (PE), plus a high rate of splanchnic and cerebral vein thrombosis.10  These patients also carry a high risk of bleeding,9  which is a very important element to consider when starting antithrombotic treatments. Older age and a history of thrombosis are well-established independent predictors of thrombosis in MPN,11,12  whereas JAK2 mutation is an independent risk factor only in ET.13  Hyperviscosity due to erythrocytosis correlates with greater thrombotic risk in PV, and a hematocrit less than 45% is protective against cardiovascular (CV) deaths.14  Leukocytosis is likely to be a risk factor, but data are not conclusive, and it is not formally included in prognostic scores.15  Risk factors for thrombosis in MPN are summarized in Table 1 (left panel).

Treatment decisions are driven by risk stratification, based on the probability to develop a thrombotic event.16  As shown in Figure 2A, low-risk patients with PV should receive primary thromboprophylaxis with low-dose aspirin (75-100 mg) once daily. This indication originates from the European Collaboration on Low-Dose Aspirin in Polycythemia Vera study results, demonstrating that aspirin reduced the risk of combined end points of nonfatal myocardial infarction, nonfatal stroke, PE, major venous thrombosis, or death from CV causes.17  Of note, aspirin was not significantly protective of major CV and venous thrombotic events taken individually. In all patients with PV, phlebotomy is recommended to maintain hematocrit less than 45%.14  Finally, in high-risk patients with PV, cytoreduction must be given to minimize the thrombotic risk.16  To date, HU is the first-line drug of choice, with a starting dose of 500 mg 2 times daily. Interferon and peginterferons have also shown to prevent thrombotic complications in patients with PV and can be considered for younger patients or pregnant women requiring cytoreduction.18  Two randomized trials Randomized Study of Efficacy and Safety in Polycythemia Vera with JAK Inhibitor INCB018424 versus Best Supportive Care (RESPONSE and RESPONSE-2) in patients with PV resistant or intolerant to HU compared the JAK1/2 inhibitor ruxolitinib to best available therapy, showing a better control in hematocrit levels and symptoms in the ruxolitinib arm.19,20  A 5-year follow-up analysis of the RESPONSE trial showed that also thrombotic complications were lower in the ruxolitinib group.21  Currently, ruxolitinib is approved as second-line therapy in high-risk patients with PV in the United States and Europe.

Figure 2.

(A) Thromboprophylaxis in PV according to risk stratification. (B) Thromboprophylaxis in ET according to risk stratification. (C) Thromboprophylaxis in pre-MF according to risk stratification. BID, twice daily; HCT, hematocrit; INF-α, interferon α; OD, once daily; WT, wild type.

Figure 2.

(A) Thromboprophylaxis in PV according to risk stratification. (B) Thromboprophylaxis in ET according to risk stratification. (C) Thromboprophylaxis in pre-MF according to risk stratification. BID, twice daily; HCT, hematocrit; INF-α, interferon α; OD, once daily; WT, wild type.

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In ET, primary thromboprophylaxis with low-dose aspirin is recommended in low- to high-risk patients, whereas very low-risk patients might not require any therapy unless in the presence of CV risk factors; cytoreduction is recommended in patients with intermediate-risk disease and CV risk factors, as well as in high-risk patients (Figure 2B). First-line drugs of choice in ET are HU and anagrelide,22  whereas interferons have proved effective in HU-intolerant/resistant patients and should be preferred in young or pregnant patients.18  In MF, the other major competing events (ie, acute leukemia transformation, infections, etc) may obscure the real incidence of thrombotic complications, and treatment is focused on symptom relief or disease eradication rather than thrombosis prevention. However, in pre-MF, in which the risk of vascular events in patients is similar to that of ET,23  a proposed pragmatic approach includes no treatment or low-dose aspirin in asymptomatic patients, aspirin or oral anticoagulation if having a positive history of arterial or venous thrombosis, and HU as first-line cytoreduction in case of thrombocytosis or leukocytosis24  (Figure 2C). Of interest, new drugs targeting specific mechanisms are becoming available, such as the antibody blocking the adhesion molecule P-selectin crizanlizumab, used to prevent vaso-occlusive crisis in sickle cell disease,25  which is under investigation in combination with ruxolitinib in MF (Platform Study of Novel Ruxolitinib Combinations in Myelofibrosis Patients, NCT04097821).

COVID-19

VTE has emerged as an important complication in hospitalized patients with COVID-19. High rates of VTE, particularly PE, have been demonstrated in both acutely ill patients, who require hospital admission without advanced clinical support, and critically ill patients, who develop respiratory or CV failure requiring advanced clinical support in the intensive care unit. In these 2 categories of patients, the incidence of VTE has been assessed to be 5% to 8% and 18% to 28%, respectively, regardless of pharmacologic thromboprophylaxis,26,27  whereas the pooled incidence of bleeding is 7.8%.27  Arterial thromboembolism, including stroke, also occurs but less frequently.28  Thrombotic complications in COVID-19 are associated with mortality.29 

Hemostatic abnormalities (ie, elevation of D-dimer and fibrinogen), dysregulated immune response, and endothelial damage are the major features of a systemic thromboinflammation process.30  Autopsy findings have revealed widespread pulmonary microthrombosis and extensive pulmonary angiogenesis, in addition to frequent extrapulmonary microthrombosis and thromboemboli, consistent with disease-specific hypercoagulability.31  Among predictors of VTE in hospitalized patients with COVID-19, elevated D-dimer levels have been associated with clotting complications and poor outcome.32,33  Older age and the development of acute respiratory distress syndrome are among clinical risk factors for VTE29  (Table 1, right panel). However, risk stratification models have not been validated in the COVID-19 setting.

Primary anticoagulant prophylaxis is required, but the optimal strategy for patients hospitalized with COVID-19 is still a matter of debate. In general, in all acutely ill hospitalized medical and critical patients, the American College of Clinical Pharmacy (ACCP) and American Society of Hematology (ASH) guidelines recommend prophylactic (low-dose) low molecular weight heparin (LMWH) or fondaparinux once daily or unfractionated heparin (UFH) subcutaneously 2 or 3 times daily, unless there are contraindications, such as active bleeding or high bleeding risk.34,35  COVID-19–specific guidelines from the major scientific societies suggest thromboprophylaxis with low- or intermediate-dose LMWH after a careful assessment of the bleeding risk (Table 2).

Low (prophylactic), intermediate (half-therapeutic), and full (therapeutic) doses of anticoagulants (mainly LMWH or UFH) have been investigated as primary thromboprophylaxis regimens in acutely ill and critically ill patients (Antithrombotic Therapy to Ameliorate Complications of COVID-19; A Randomised, Embedded, Multi-factorial, Adaptive Platform Trial for Community-Acquired Pneumonia; Accelerating COVID-19 Therapeutic Interventions and Vaccines 4 ACUTE, Inspiration trials). New data showing the superiority of therapeutic doses in the acutely ill setting have recently emerged.41  On the contrary, in critically ill patients, therapeutic and intermediate-intensity anticoagulation have not shown advantages compared with low-dose thromboprophylaxis, while increasing significantly the risk of bleeding and thrombocytopenia42  (R. Zarychanski, unpublished data). The guidelines are constantly evolving and updating as the results of ongoing trials become available.

Among other thromboprophylaxis methods, there is the rationale for potential benefit of antiplatelet agents, but only 6 studies have involved these compounds,37  and 1 has shown a potential benefit of aspirin, not of other antiplatelet drugs, in preventing COVID-19 fatal course.43 

MPN plus COVID-19

Given the high thrombotic risk of both MPN and COVID-19, it is quite natural to wonder how patients with MPN deal with the SARS-CoV-2 infection. The recent retrospective MPN-COVID trial, promoted by the European LeukemiaNet, found a cumulative incidence of 8.6% thrombosis in hospitalized patients with MPN and COVID-19.7  Thrombotic events were mainly venous (VTE = 7.4% vs arterial thromboembolism = 1.9%), suggesting a closer link to infection rather than MPN (in which arterial events are more common), and were significantly more frequent in ET than in PV or MF. Data on thromboprophylaxis with various regimens of LMWH were very heterogeneous.

In March 2020 (as well as now), there was no specific evidence on which dose of LMWH had a most favorable risk/efficacy profile in MPN/COVID-19. Therefore, in practice, the dose could only be chosen on an empirical basis, evaluating the presence of generic vascular risk factors.

CLINICAL CASE (continued)

For VTE prophylaxis, we adopted the ACCP and ASH guidelines' recommendations for acutely ill medical patients,34,35  and LMWH enoxaparin 4000 IU subcutaneously daily was started. Treatment with aspirin was also continued due to the patient's history of transient ischemic attack. Ruxolitinib was reduced to 5 mg twice a day, given the possible drug-drug interaction with lopinavir/ritonavir, as it is known that the antiviral can interfere with JAK inhibitor metabolism, slowing down its excretion and enhancing toxicity.

The patient's clinical condition improved with treatment. However, 3 days after admission, he developed epistaxis. Both aspirin and anticoagulation were withdrawn, and bleeding stopped in 1 day. Only prophylactic LMWH was resumed.

Three days later, he suddenly became significantly more breathless—his oxygen saturation was now 75% on air. Computed tomography pulmonary angiography showed widespread bilateral ground-glass opacities consistent with extensive COVID-19 pneumonitis plus bilateral segmental pulmonary emboli. No DVT of legs was detected by ultrasound.

Blood tests showed the typical COVID-19 profile with mild lymphopenia, mildly raised alanine aminotransferase and lactate dehydrogenase, modestly raised C-reactive protein, raised ferritin, and markedly raised D-dimer.

He was put on high-flow oxygen, and therapeutic-dose LMWH enoxaparin 1 mg/kg twice daily was started. He did not require mechanical ventilation or intensive care unit management.

Treatment of VTE, including DVT and PE, is divided in 3 phases: acute (first 5-10 days), long term (from end of acute treatment to 3-6 months), and extended (beyond 3-6 months). Anticoagulation with therapeutic-dose LMWH or fondaparinux is recommended over UFH intravenously or subcutaneously for initial treatment, before switching to a long-term anticoagulation regimen.44-46  This schema is not different for patients with MPN10  or COVID-19.

Before starting anticoagulant therapy, a careful assessment of the bleeding risk of both patients with MPN9  and COVID-1927  is required. In addition, an increased risk of heparin-induced thrombocytopenia in MPN calls for special care in monitoring the patient platelet count during the heparin course.47 

CLINICAL CASE (continued)

In the initial phase, we opted for therapeutic doses of LMWH as long as the patient was hospitalized.

After 10 days, the patient's condition improved, and antiviral drug was stopped. However, the patient's blood counts had worsened from admission, with mild thrombocytopenia (110 × 109/L), hemoglobin of 110 g/L, and white blood cell count of 5 × 109/L. Ruxolitinib suspension was evaluated, also considering the need to keep the patient on anticoagulation.

The management of MPN-directed cytoreductive therapy after the onset of SARS-CoV-2 infection has been questioned since the beginning of the pandemic, and expert consensus platforms appointed by ASH have been issued (https://www.hematology.org/COVID-19/COVID-19-and-myeloproliferativeneoplasms). The results from an observational study promoted by the European LeukemiaNet group, focusing on clinical/laboratory presentation and risk factors for overall survival in patients with MPN during the acute phase of SARS-CoV-2 infection, show a high mortality risk among patients with MPN (28.6%), especially in MF, and demonstrate that withdrawal of ruxolitinib was a negative prognostic factor.48 

Abrupt suspension of ruxolitinib appears associated with a “ruxolitinib discontinuation syndrome,”49  with debilitation, progressive splenomegaly, or, rarely, cytokine storm. Moreover, reports from observational studies suggest that JAK inhibitors may be beneficial for critically ill patients with COVID-19,50  although these results have not been confirmed in a large randomized commercial study (Study to Assess the Efficacy and Safety of Ruxolitinib in Patients With COVID-19 Associated Cytokine Storm, NCT04362137; results on clinicaltrials.gov). Adjustments of other cytoreductive drugs (ie, HU, interferon, or anagrelide) are not recommended in patients with MPN and COVID-19 (https://www.hematology.org/COVID-19/COVID-19-and-myeloproliferativeneoplasms).

CLINICAL CASE (continued)

We opted to keep the patient on reduced-dose ruxolitinib, strictly monitored the laboratory and clinical parameters, and contemplated that the infection state and the concomitant medications could in part be responsible for the mild pancytopenia observed. Indeed, blood values did not further decrease, and a slight improvement in platelet count was observed apart from antiviral suspension.

The patient recovered sufficiently to be discharged home, and long-term anticoagulant and PV-specific treatments were planned.

The aim of long-term anticoagulation (3-6 months) is to prevent thrombosis recurrence. DVT of legs or PE in patients with MPN should be treated the same as DVT or PE occurring in patients without MPN. Therefore, continuation of anticoagulant therapy is based on evaluation of the underlying risk factors for VTE recurrence.44,46  This risk in MPN, particularly in patients with PV, is significantly higher than in the general population.51  Long-term and, possibly, extended (after 6 months) anticoagulation is recommended in MPN due to the presence of a permanent risk factor, such as a chronic malignant disease. Other strong predictors for recurrence in MPN are age older than 60 years and history of thrombosis.

The risk of VTE recurrence in COVID-19 is not well defined. Recent data show that postdischarge thromboembolic outcomes and mortality may be frequent after COVID-19 hospitalization, and anticoagulation may reduce this risk by 46%,52  but these results are controversial.53,54  Currently, postdischarge thromboprophylaxis is not recommended.

Concerning the type of long-term anticoagulant regimen, we can choose among the following oral agents: vitamin K antagonists (VKAs) aiming for targeting an international normalized ratio of 2.5 (range, 2.0-3.0) or direct oral anticoagulants (DOACs).45,46 

So far, after acute treatment with LMWH, early initiation of VKAs is the most used strategy in patients with MPN.

At present, there are no evidence-based data on the efficacy and safety of DOACs in patients with MPN with VTE. Data from a randomized controlled trial conducted in noncancer and cancer populations have shown that these drugs are as effective as VKAs, with a lower risk of bleeding. In addition, DOACs do not need laboratory monitoring and have less dietary interference, and therefore, they have become widely used in clinical practice. Small series of patients with MPN treated with DOACs have been published.1  Of interest, recently, the results of a large retrospective study of 442 patients with MPN receiving DOACs (factor Xa inhibitors) or VKAs either for VTE or atrial fibrillation confirm a similar risk/benefit profile of both regimens for treatment of VTE in MPN.55 

CLINICAL CASE (continued)

For long-term home anticoagulant treatment, we chose to stop LMWH and shift to therapy with a DOAC, based on our personal experience with these drugs and because of their easy handling, particularly with the concurrent difficulties due to the pandemic and hospital crash. I discussed the 2 options with the patient, and he shared my decision and preferred DOAC. He started oral rivaroxaban 20 mg daily.

After 2 weeks from discharge, the patient was visited as an outpatient. Blood counts had recovered, so we decided to resume ruxolitinib at the regular dose of 10 mg twice daily.

After a 6-month follow-up, he was doing well, with no recurrence of VTE.

In conclusion, MPN and COVID-19 are both high-risk conditions for thrombosis. Thus, it is likely that patients with MPN, particularly patients with PV and ET, are more prone to develop thrombotic complications during SARS-CoV-2 infection. In this work, the journey is illustrated by a 71-year-old patient with PV who faced an acute infectious illness, COVID-19, during his chronic disease and related treatment. Not only did he have to deal with hospitalization and COVID-19 treatments, but despite thromboprophylaxis, he also experienced a PE. As a consequence, all tasks related to the choice and duration of anticoagulation in the acute phase and during the long-term postdischarge, as well as the issues related to the PV-specific treatments, had to be handled.

There is a clear need to protect patients with MPN from SARS- CoV-2 infection and its complications. Nowadays, vaccination represents our best weapon against COVID-19. Although an endogenous production of anti-PF4/heparin antibodies, also involved in the pathogenesis of vaccine-induced thrombotic thrombocytopenia,56  has been described in patients with MPN,57  no cases of vaccine-induced thrombotic thrombocytopenia syndrome associated with MPN have been published so far.58,59  A recent small cohort study on chronic myeloid neoplasms showed an impaired seroconversion after vaccination, especially in patients receiving ruxolitinib,60  but larger studies with a longer follow-up are required to confirm these findings. Moreover, there are reassuring data concerning the efficacy of the COVID-19 mRNA vaccine in the general MPN population.61,62  Therefore, at this time, patients with MPN should be encouraged to receive vaccination against COVID-19.

The author thanks doctors Francesca Schieppati and Marina Marchetti for invaluable assistance and critical review of the manuscript.

Anna Falanga discloses no relevant conflicts of interest.

Anna Falanga: nothing to disclose.

1.
Falanga
A
,
Marchetti
M
,
Schieppati
F
.
Prevention and management of thrombosis in BCR/ABL-negative myeloproliferative neoplasms
.
Hamostaseologie
.
2021
;
41
(
1
):
48
-
57
.
doi:10.1055/a-1334-3259
.
2.
Connors
JM
,
Levy
JH
.
COVID-19 and its implications for thrombosis and anticoagulation
.
Blood
.
2020
;
135
(
23
):
2033
-
2040
.
doi:10.1182/blood.2020006000
.
3.
Falanga
A
,
Marchetti
M
.
Thrombosis in myeloproliferative neoplasms
.
Semin Thromb Hemost
.
2014
;
40
(
3
):
348
-
358
.
doi:10.1055/s-0034-1370794
.
4.
Guy
A
,
Gourdou-Latyszenok
V
,
Le Lay
N
, et al.
Vascular endothelial cell expression of JAK2V617F is sufficient to promote a pro-thrombotic state due to increased P-selectin expression
.
Haematologica
.
2019
;
104
(
1
):
70
-
81
.
doi:10.3324/haematol.2018.195321
.
5.
Joly
BS
,
Siguret
V
,
Veyradier
A
.
Understanding pathophysiology of hemostasis disorders in critically ill patients with COVID-19
.
Intensive Care Med
.
2020
;
46
(
8
):
1603
-
1606
.
doi:10.1007/s00134-020-06088-1
.
6.
Marchetti
M
,
Gomez-Rosas
P
,
Sanga
E
, et al.
Endothelium activation markers in severe hospitalized COVID-19 patients: role in mortality risk prediction
.
TH Open
.
2021
;
5
(
3
):
e253
-
e263
.
doi:10.1055/s-0041-1731711
.
7.
Barbui
T
,
De Stefano
V
,
Alvarez-Larran
A
, et al.
Among classic myeloproliferative neoplasms, essential thrombocythemia is associated with the greatest risk of venous thromboembolism during COVID-19
.
Blood Cancer J
.
2021
;
11
(
2
):
21
.
doi:10.1038/s41408-021-00417-3
.
8.
Hultcrantz
M
,
Björkholm
M
,
Dickman
PW
, et al.
Risk for arterial and venous thrombosis in patients with myeloproliferative neoplasms: a population-based cohort study
.
Ann Intern Med
.
2018
;
168
(
5
):
317
-
325
.
doi:10.7326/M17-0028
.
9.
Rungjirajittranon
T
,
Owattanapanich
W
,
Ungprasert
P
,
Siritanaratkul
N
,
Ruchutrakool
T
.
A systematic review and meta-analysis of the prevalence of thrombosis and bleeding at diagnosis of Philadelphia-negative myeloproliferative neoplasms
.
BMC Cancer
.
2019
;
19
(
1
):
184
.
doi:10.1186/s12885-019-5387-9
.
10.
De Stefano
V
,
Finazzi
G
,
Barbui
T
.
Antithrombotic therapy for venous thromboembolism in myeloproliferative neoplasms
.
Blood Cancer J
.
2018
;
8
(
7
):
65
.
doi:10.1038/s41408-018-0101-8
.
11.
Marchioli
R
,
Finazzi
G
,
Landolfi
R
, et al.
Vascular and neoplastic risk in a large cohort of patients with polycythemia vera
.
J Clin Oncol
.
2005
;
23
(
10
):
2224
-
2232
.
doi:10.1200/JCO.2005.07.062
.
12.
Carobbio
A
,
Thiele
J
,
Passamonti
F
, et al.
Risk factors for arterial and venous thrombosis in WHO-defined essential thrombocythemia: an international study of 891 patients
.
Blood
.
2011
;
117
(
22
):
5857
-
5859
.
doi:10.1182/blood-2011-02-339002
.
13.
Dahabreh
IJ
,
Zoi
K
,
Giannouli
S
,
Zoi
C
,
Loukopoulos
D
,
Voulgarelis
M
.
Is JAK2 V617F mutation more than a diagnostic index? A meta-analysis of clinical outcomes in essential thrombocythemia
.
Leuk Res
.
2009
;
33
(
1
):
67
-
73
.
doi:10.1016/j.leukres.2008.06.006
.
14.
Marchioli
R
,
Finazzi
G
,
Specchia
G
, et al
;
CYTO-PV Collaborative Group
.
Cardiovascular events and intensity of treatment in polycythemia vera
.
N Engl J Med
.
2013
;
368
(
1
):
22
-
33
.
doi:10.1056/NEJMoa1208500
.
15.
Carobbio
A
,
Ferrari
A
,
Masciulli
A
,
Ghirardi
A
,
Barosi
G
,
Barbui
T
.
Leukocytosis and thrombosis in essential thrombocythemia and polycythemia vera: a systematic review and meta-analysis
.
Blood Adv
.
2019
;
3
(
11
):
1729
-
1737
.
doi:10.1182/bloodadvances.2019000211
.
16.
Tefferi
A
,
Barbui
T
.
Polycythemia vera and essential thrombocythemia: 2021 update on diagnosis, risk-stratification and management
.
Am J Hematol
.
2020
;
95
(
12
):
1599
-
1613
.
doi:10.1002/ajh.26008
.
17.
Landolfi
R
,
Marchioli
R
,
Kutti
J
, et al
;
European Collaboration on Low-Dose Aspirin in Polycythemia Vera Investigators
.
Efficacy and safety of low-dose aspirin in polycythemia vera
.
N Engl J Med
.
2004
;
350
(
2
):
114
-
124
.
doi:10.1056/NEJMoa035572
.
18.
Mesa
RA
,
Jamieson
C
,
Bhatia
R
, et al.
NCCN guidelines insights: myeloproliferative neoplasms, version 2.2018
.
J Natl Compr Canc Netw
.
2017
;
15
(
10
):
1193
-
1207
.
doi:10.6004/jnccn.2017.0157
.
19.
Vannucchi
AM
,
Kiladjian
JJ
,
Griesshammer
M
, et al.
Ruxolitinib versus standard therapy for the treatment of polycythemia vera
.
N Engl J Med
.
2015
;
372
(
5
):
426
-
435
.
doi:10.1056/NEJMoa1409002
.
20.
Passamonti
F
,
Griesshammer
M
,
Palandri
F
, et al.
Ruxolitinib for the treatment of inadequately controlled polycythaemia vera without splenomegaly (RESPONSE-2): a randomised, open-label, phase 3b study
.
Lancet Oncol
.
2017
;
18
(
1
):
88
-
99
.
doi:10.1016/S1470-2045(16)30558-7
.
21.
Kiladjian
J-J
,
Zachee
P
,
Hino
M
, et al.
Long-term efficacy and safety of ruxolitinib versus best available therapy in polycythaemia vera (RESPONSE): 5-year follow up of a phase 3 study
.
Lancet Haematol
.
2020
;
7
(
3
):
e226
-
e237
.
doi:10.1016/S2352-3026(19)30207-8
.
22.
Gisslinger
H
,
Gotic
M
,
Holowiecki
J
, et al
;
ANAHYDRET Study Group
.
Anagrelide compared with hydroxyurea in WHO-classified essential thrombocythemia: the ANAHYDRET Study, a randomized controlled trial
.
Blood
.
2013
;
121
(
10
):
1720
-
1728
.
doi:10.1182/blood-2012-07-443770
.
23.
Rumi
E
,
Boveri
E
,
Bellini
M
, et al
;
Associazione Italiana per la Ricerca sul Cancro Gruppo Italiano Malattie Mieloproliferative Investigators
.
Clinical course and outcome of essential thrombocythemia and prefibrotic myelofibrosis according to the revised WHO 2016 diagnostic criteria
.
Oncotarget
.
2017
;
8
(
60
):
101735
-
101744
.
doi:10.18632/oncotarget.21594
.
24.
Finazzi
G
,
Vannucchi
AM
,
Barbui
T
.
Prefibrotic myelofibrosis: treatment algorithm 2018
.
Blood Cancer J
.
2018
;
8
(
11
):
104
.
doi:10.1038/s41408-018-0142-z
.
25.
Ataga
KI
,
Kutlar
A
,
Kanter
J
, et al.
Crizanlizumab for the prevention of pain crises in sickle cell disease
.
N Engl J Med
.
2017
;
376
(
5
):
429
-
439
.
doi:10.1056/NEJMoa1611770
.
26.
Nopp
S
,
Moik
F
,
Jilma
B
,
Pabinger
I
,
Ay
C
.
Risk of venous thromboembolism in patients with COVID-19: a systematic review and meta–analysis
.
Res Pr Thromb Haemost
.
2020
;
4
(
7
):
1178
-
1191
.
doi:10.13039/501100002428
.
27.
Jiménez
D
,
García-Sanchez
A
,
Rali
P
, et al.
Incidence of VTE and bleeding among hospitalized patients with coronavirus disease 2019: a systematic review and meta-analysis
.
Chest
.
2021
;
159
(
3
):
1182
-
1196
.
doi:10.1016/j.chest.2020.11.005
.
28.
Fara
MG
,
Stein
LK
,
Skliut
M
,
Morgello
S
,
Fifi
JT
,
Dhamoon
MS
.
Macrothrombosis and stroke in patients with mild Covid-19 infection
.
J Thromb Haemost
.
2020
;
18
(
8
):
2031
-
2033
.
doi:10.1111/jth.14938
.
29.
Cui
S
,
Chen
S
,
Li
X
,
Liu
S
,
Wang
F
.
Prevalence of venous thromboembolism in patients with severe novel coronavirus pneumonia
.
J Thromb Haemost
.
2020
;
18
(
6
):
1421
-
1424
.
doi:10.1111/jth.14830
.
30.
Iba
T
,
Levy
JH
,
Levi
M
,
Thachil
J
.
Coagulopathy in COVID-19
.
J Thromb Haemost
.
2020
;
18
(
9
):
2103
-
2109
.
doi:10.1111/jth.14975
.
31.
Fahmy
OH
,
Daas
FM
,
Salunkhe
V
, et al.
Is microthrombosis the main pathology in coronavirus disease 2019 severity? A systematic review of the postmortem pathologic findings
.
Crit Care Explor
.
2021
;
3
(
5
):
e0427
.
doi:10.1097/cce.0000000000000427
.
32.
Mouhat
B
,
Besutti
M
,
Bouiller
K
, et al.
Elevated D-dimers and lack of anticoagulation predict PE in severe COVID-19 patients
.
Eur Respir J
.
2020
;
56
(
4
):
2001811
.
doi:10.1183/13993003.01811-2020
.
33.
Al-Samkari
H
,
Karp Leaf
RS
,
Dzik
WH
, et al.
COVID-19 and coagulation: bleeding and thrombotic manifestations of SARS-CoV-2 infection
.
Blood
.
2020
;
136
(
4
):
489
-
500
.
doi:10.1182/blood.2020006520
.
34.
Kahn
SR
,
Lim
W
,
Dunn
AS
, et al.
Prevention of VTE in nonsurgical patients: Antithrombotic therapy and prevention of thrombosis, 9th ed: American College of Chest Physicians Evidence-Based Clinical Practice Guidelines
.
Chest
.
2012
;
141
(
2, suppl
):
e195S
-
e226S
.
doi:10.1378/chest.11-2296
.
35.
Schünemann
HJ
,
Cushman
M
,
Burnett
AE
, et al.
American Society of Hematology 2018 guidelines for management of venous thromboembolism: prophylaxis for hospitalized and nonhospitalized medical patients
.
Blood Adv
.
2018
;
2
(
22
):
3198
-
3225
.
doi:10.1182/bloodadvances.2018022954
.
36.
Moores
LK
,
Tritschler
T
,
Brosnahan
S
, et al.
Prevention, diagnosis, and treatment of VTE in patients with coronavirus disease 2019: CHEST Guideline and Expert Panel Report
.
Chest
.
2020
;
158
(
3
):
1143
-
1163
.
doi:10.1016/j.chest.2020.05.559
.
37.
Barnes
GD
,
Burnett
A
,
Allen
A
, et al.
Thromboembolism and anticoagulant therapy during the COVID-19 pandemic: interim clinical guidance from the anticoagulation forum
.
J Thromb Thrombolysis
.
2020
;
50
(
1
):
72
-
81
.
doi:10.1007/s11239-020-02138-z
.
38.
Cuker
A
,
Tseng
EK
,
Nieuwlaat
R
, et al.
American Society of Hematology 2021 guidelines on the use of anticoagulation for thromboprophylaxis in patients with COVID-19
.
Blood Adv
.
2021
;
5
(
3
):
872
-
888
.
doi:10.1182/bloodadvances.2020003763
.
39.
Spyropoulos
AC
,
Levy
JH
,
Ageno
W
, et al
;
Subcommittee on Perioperative, Critical Care Thrombosis, Haemostasis of the Scientific, Standardization Committee of the International Society on Thrombosis and Haemostasis
.
Scientific and standardization committee communication: clinical guidance on the diagnosis, prevention, and treatment of venous thromboembolism in hospitalized patients with COVID-19
.
J Thromb Haemost
.
2020
;
18
(
8
):
1859
-
1865
.
doi:10.1111/jth.14929
.
40.
Marietta
M
,
Ageno
W
,
Artoni
A
, et al.
Position paper from Italian Society on Thrombosis and Haemostasis (SISET)
.
Blood Transfus
.
2020
;
18
(
3
):
167
-
169
.
doi:10.2450/2020.0083-20
.
41.
Lawler
PR
, et al.
Therapeutic anticoagulation in non-critically ill patients with Covid-19
.
NEJM
.
2021
Aug
26
;
385
(
9
):
790
-
802
.
doi:10.1001/jama.2021.4152
.
42.
Sadeghipour
P
,
Talasaz
AH
,
Rashidi
F
, et al.
Effect of intermediate-dose vs standard-dose prophylactic anticoagulation on thrombotic events, extracorporeal membrane oxygenation treatment, or mortality among patients with COVID-19 admitted to the intensive care unit: the INSPIRATION randomized clinical trial
.
JAMA
.
2021
;
325
(
16
):
1620
-
1630
.
doi:10.1001/jama.2021.4152
.
43.
Tremblay
D
,
van Gerwen
M
,
Alsen
M
, et al.
Impact of anticoagulation prior to COVID-19 infection: a propensity score-matched cohort study
.
Blood
.
2020
;
136
(
1
):
144
-
147
.
doi:10.1182/blood.2020006941
.
44.
Kearon
C
,
Akl
EA
,
Comerota
AJ
, et al.
Antithrombotic therapy for VTE disease: antithrombotic therapy and prevention of thrombosis, 9th ed: American College of Chest Physicians Evidence-Based Clinical Practice Guidelines
.
Chest
.
2012
;
141
(
2, suppl
):
e419S
-
e496S
.
doi:10.1378/chest.11-2301
.
45.
Kearon
C
,
Akl
EA
,
Ornelas
J
, et al.
Antithrombotic therapy for VTE disease: CHEST guideline and expert panel report
.
Chest
.
2016
;
149
(
2
):
315
-
352
.
doi:10.1016/j.chest.2015.11.026
.
46.
Ortel
TL
,
Neumann
I
,
Ageno
W
, et al.
American Society of Hematology 2020 guidelines for management of venous thromboembolism: treatment of deep vein thrombosis and pulmonary embolism
.
Blood Adv
.
2020
;
4
(
19
):
4693
-
4738
.
doi:10.1182/bloodadvances.2020001830
.
47.
Castelli
R
,
Gallipoli
P
,
Schiavon
R
,
Teatini
T
,
Deliliers
GL
,
Bergamaschini
L
.
High prevalence of heparin induced thrombocytopenia with thrombosis among patients with essential thrombocytemia carrying V617F mutation
.
J Thromb Thrombolysis
.
2018
;
45
(
1
):
106
-
113
.
doi:10.1007/s11239-017-1566-1
.
48.
Barbui
T
,
Vannucchi
AM
,
Alvarez-Larran
A
, et al.
High mortality rate in COVID-19 patients with myeloproliferative neoplasms after abrupt withdrawal of ruxolitinib
.
Leukemia
.
2021
;
35
(
2
):
485
-
493
.
doi:10.1038/s41375-020-01107-y
.
49.
Coltro
G
,
Mannelli
F
,
Guglielmelli
P
,
Pacilli
A
,
Bosi
A
,
Vannucchi
AM
.
A life-threatening ruxolitinib discontinuation syndrome
.
Am J Hematol
.
2017
;
92
(
8
):
833
-
838
.
doi:10.1002/ajh.24775
.
50.
D'Alessio
A
,
Del Poggio
P
,
Bracchi
F
, et al.
Low-dose ruxolitinib plus steroid in severe SARS-CoV-2 pneumonia
.
Leukemia
.
2021
;
35
(
2
):
635
-
638
.
doi:10.1038/s41375-020-01087-z
.
51.
De Stefano
V
,
Ruggeri
M
,
Cervantes
F
, et al.
High rate of recurrent venous thromboembolism in patients with myeloproliferative neoplasms and effect of prophylaxis with vitamin K antagonists
.
Leukemia
.
2016
;
30
(
10
):
2032
-
2038
.
doi:10.1038/leu.2016.85
.
52.
Giannis
D
,
Allen
SL
,
Tsang
J
, et al.
Postdischarge thromboembolic outcomes and mortality of hospitalized patients with COVID-19: the CORE-19 registry
.
Blood
.
2021
;
137
(
20
):
2838
-
2847
.
doi:10.1182/blood.2020010529
.
53.
Rashidi
F
,
Barco
S
,
Kamangar
F
, et al.
Incidence of symptomatic venous thromboembolism following hospitalization for coronavirus disease 2019: prospective results from a multi-center study
.
Thromb Res
.
2021
;
198
:
135
-
138
.
doi:10.1016/j.thromres.2020.12.001
.
54.
Engelen
MM
,
Vandenbriele
C
,
Balthazar
T
, et al.
Venous thromboembolism in patients discharged after COVID-19 hospitalization
.
Semin Thromb Hemost
.
2021
;
47
(
4
):
362
-
371
.
doi:10.1055/s-0041-1727284
.
55.
Barbui
T
,
De Stefano
V
,
Carobbio
A
, et al.
Direct oral anticoagulants for myeloproliferative neoplasms: results from an international study on 442 patients
.
Leukemia
.
2021
;
35
:
2989
-
2993
.
doi:10.1038/s41375-021-01279-1
.
56.
Scully
M
,
Singh
D
,
Lown
R
, et al.
Pathologic antibodies to platelet factor 4 after ChAdOx1 nCoV-19 vaccination
.
N Engl J Med
.
2021
;
384
(
23
):
2202
-
2211
.
doi:10.1056/NEJMoa2105385
.
57.
Meyer
SC
,
Steinmann
E
,
Lehmann
T
, et al.
Anti-platelet factor 4/heparin antibody formation occurs endogenously and at unexpected high frequency in polycythemia vera
.
Biomed Res Int
.
2017
;
2017
:
9876819
.
doi:10.1155/2017/9876819
.
58.
Palandri
F
,
Breccia
M
,
De Stefano
V
,
Passamonti
F
.
Philadelphia-negative chronic myeloproliferative neoplasms during the COVID-19 pandemic: challenges and future scenarios
.
Cancers (Basel)
.
2021
;
13
(
19
):
4750
.
doi:10.3390/cancers13194750
.
59.
Gresele
P
,
Momi
S
,
Marcucci
R
,
Ramundo
F
,
De Stefano
V
,
Tripodi
A
.
Interactions of adenoviruses with platelets and coagulation and the vaccine-associated autoimmune thrombocytopenia thrombosis syndrome
.
Haematologica
.
2021
;
106
(
12
):
3034
-
3045
.
doi:10.3324/haematol.2021.279289
.
60.
Chowdhury
O
,
Bruguier
H
,
Mallett
G
, et al.
Impaired antibody response to COVID-19 vaccination in patients with chronic myeloid neoplasms
.
Br J Haematol
.
2021
;
194
(
6
):
1010
-
1015
.
doi:10.1111/bjh.17644
.
61.
Pimpinelli
F
,
Marchesi
F
,
Piaggio
G
, et al.
Fifth-week immunogenicity and safety of anti-SARS-CoV-2 BNT162b2 vaccine in patients with multiple myeloma and myeloproliferative malignancies on active treatment: preliminary data from a single institution
.
J Hematol Oncol
.
2021
;
14
(
1
):
81
.
doi:10.1186/s13045-021-01090-6
.
62.
Harrington
P
,
de Lavallade
H
,
Doores
KJ
, et al.
Single dose of BNT162b2 mRNA vaccine against SARS-CoV-2 induces high frequency of neutralising antibody and polyfunctional T-cell responses in patients with myeloproliferative neoplasms
.
Leukemia
.
2021
;
35
:
3573
-
3577
.
doi:10.1038/s41375-021-01300-7
.