Mono-OKT3-Fab copotentiation primarily enhances expansion of top-ranked T-cell clones. (A-D) TCR clones for each exog-NLV-bulk-responsive donor were ranked from most abundant to least abundant for each condition. Differences in rank-vs-rank performance concentrated in the top 10 clones. (E-H) Among top-ranked clones, there was variability in the extent to which clones were amplified by exogenous NLV, mono-OKT3-Fab, or both in combination. The top 10 ranked clones from the NLV + mono-OKT3-Fab condition are shown with their corresponding live-cell number abundance in the other 3 conditions. Boxed amino acid sequences indicate NLV-specific clones (clones with greater abundance in NLV + Ms-IgG-Fab vs no exogenous peptide + Ms-IgG-Fab condition or, for public TCRs, observation of that pattern in ≥1 other donor or previously reported in the literature). Sequences in bold represent public TCR-bearing clones appearing in multiple donors in the present study or previously reported in the literature. Heatmaps visualize the increase in clonal cell number generated by exogenous NLV, mono-OKT3-Fab, or both in combination.