Integrated mRNA and protein profiling of AML cells lines with missense and truncating TP53 mutations. (A) Volcano plot (R package version 1.4.0) showing DE mRNA species and proteins between AML cell lines with missense (Kasumi-1 cells; p.R248Q; Broad Institute Cancer Cell Line Encyclopedia) and truncating (splice site) mutations of TP53 (KG-1 cells).³⁰  (B) Heatmap of the top DE mRNA species and proteins between KG-1 AML and Kasumi-1 AML (unsupervised hierarchical clustering; Euclidean distance; complete linkage). ClustVis, an online tool for clustering of multivariate data, was used for data analysis and visualization.⁴⁷  (C) Heatmap of signaling pathway scores in KG-1 and Kasumi-1 cells (unsupervised hierarchical clustering; Euclidean distance; complete linkage). Signature scores were calculated as detailed in "Materials and methods." (D) Kaplan-Meier estimate of survival from diagnosis in TCGA-AML cases with abnormalities in DE genes between KG-1 (n = 34) and Kasumi-1 cells (n = 10). KM curves (median split of signature scores) were generated using GEPIA2, an enhanced Web server for TCGA gene expression profiling and interactive analysis (http://gepia2.cancer-pku.cn/#index). Signature scores are calculated as the mean value of log₂ transcripts per million. GEPIA2 uses the log-rank (Mantel-Cox) test to compare survival distributions.