CBU lymphocyte content and chimerism. (A) CBU precryopreservation lymphocyte cell dose (×10⁶ per kilogram) and chimerism CBU complete blood count with differential were obtained before cryopreservation, as per current practice, and the total lymphocyte content of the CBU, as well as the lymphocyte cell dose (per kilogram of patient weight) were calculated. The orange symbols indicate the precryopreservation lymphocyte doses of patients who had CBU-derived chimerism detected after treatment, whereas the blue symbols indicate the cell doses of the patients who did not have detectable chimerism. The black horizontal lines indicate the medians for each group. (B) CBU postthaw CD3 cell dose (×10⁶ per kilogram) and chimerism CBU evaluation by flow cytometry was performed after thawing, following standard practice, and the CD3⁺ cell doses were calculated. The orange symbols indicate the postthaw CD3⁺ cell doses of patients who had CBU-derived chimerism detected after treatment, whereas the blue symbols indicate the cell doses of the patients who did not have detectable chimerism. The horizontal black lines indicate the medians for each group.