Step 1: Individual digestion of genomic DNA (500ng) with Nsp I and Sty I restriction enzymes (RE). Step 2: Ligation of digested products to corresponding adaptors. Step 3: Preferential amplification of adaptor-ligated RE digested DNA fragments with generic primer to generate products of sizes from 200 to 1100 bp. Step 4: Combination of PCR products from each RE digest. Step 5: Purification of products using polystyrene beads. Step 5: Fragmentation and labeling of the amplified DNA. Step 6: Hybridization of labeled fragments to SNP chip.

Step 1: Individual digestion of genomic DNA (500ng) with Nsp I and Sty I restriction enzymes (RE). Step 2: Ligation of digested products to corresponding adaptors. Step 3: Preferential amplification of adaptor-ligated RE digested DNA fragments with generic primer to generate products of sizes from 200 to 1100 bp. Step 4: Combination of PCR products from each RE digest. Step 5: Purification of products using polystyrene beads. Step 5: Fragmentation and labeling of the amplified DNA. Step 6: Hybridization of labeled fragments to SNP chip.

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