TNFR1 and TNFR2 are required for birinapant response. (A) Schematic diagram of CRISPR in vivo selection. Total engraftment (purple) and TNFR2ko BFP-positive (B) or TNFR1ko mCherry-positive (C) cells over the total engraftment for R-03 PDX cells. Data are representative of 3 mice per experiment. (D) In vitro birinapant response of WT, TNFR2ko, and TNFR1ko R-03 cells treated for 48 hours. (E) In vitro birinapant response after reconstitution of TNFR2 expression in TNFR2ko ALL cells. The same experiments were performed for PDX samples VHR-10 (E-H) and SR-13 (G-J), with 1 mouse per experiment (E-F,H-I). In vitro data (D,H,K) are derived from 3 independent experiments in triplicates; data are mean ± SEM. R, relapse.