Validation of association between TNFR2 and sensitivity to birinapant in an independent cohort. (A) Expression of TNFR1 and TNFR2 from the validation cohort (n = 44), with selected patients marked in blue (high TNFR2, n = 5) and red (low TNFR2, n = 5). (B) Response to birinapant for selected patients (n = 10). (C) Birinapant IC₅₀ values. (D) Rescue from birinapant (48 hours, 50 nM) with zVAD (25 μM) and/or Nec-1s (25 μM). All quantifications are mean ± SEM and were derived from 1 experiment in triplicate for the indicated number of primary samples.