Tip60 regulates Myc target genes. (A) A plot (left) of FDR vs normalized enrichment score (NES) based on GSEA from RNA-seq data of control (Tip60f/f) vs Tip60Δ/Δ LSK cells. Distribution of all transcription factor target gene sets obtained from the MSigDB (c2 and c3) is shown. The red dots indicate the gene sets for Myc and the gray dots, the non-Myc transcription factors. The dashed line represents the FDR cutoff. List of enriched Myc-associated gene sets (right). (B) Representative GSEA plots for Myc target gene sets. (C) Correlation analysis of genome-wide occupancy for Tip60 and 10 transcriptional factors (c-Myc, Tal1, Gata2, Lyl1, Lmo2, Runx1, Fli1, Meis1, Gfi1b, and Spi1) in a murine hematopoietic progenitor cell line, HPC-7, using NPMI. The color intensity represents the correlation efficiency of each 2 transcriptional factors. A total of 3651 c-Myc binding peaks, as well as 4 347 Tip60, 7 596 Tal1, 2 796 Gata2, 3 432 Lyl1, 5 202 Lmo2, 5 992 Runx1, 18 796 Fli1, 7 690 Meis1, 3 279 Gfi1b, and 18 249 Spi1 (PU.1) peaks were identified. (D) Cells of the hematopoietic progenitor cell line 32D were transduced with FLAG-TIP60 plasmid (left). Cell lysates were subjected to immunoprecipitation with FLAG-M2 beads. Proteins present in immunoprecipitates or cell lysates (input) were separated by sodium dodecyl sulfate-polyacrylamide gel electrophoresis and immunoblotted with antibodies for FLAG and c-Myc. Interaction was confirmed by reciprocal coimmunoprecipitation (right).