Genetic deletion of Tip60 leads to fetal hematopoietic failure. (A) The genotype distribution of offspring and embryos derived from extensive Tip60^f/f × Tip60^f/+; Vav-iCre breeding. (B) Representative images of Tip60^f/+ and Tip60^Δ/Δ (Tip60^f/f; Vav-iCre) embryos at E14.5 and E17.5. (C) Average absolute number of nucleated cells from control and Tip60^Δ/Δ fetal liver at different gestational ages (n = 3-5 embryos for each genotype for each gestational age). (D) Representative dot plots of flow cytometry analysis of control and Tip60^Δ/Δ E14.5 fetal liver cells and frequencies of the gated populations. (E) Absolute number of HSPC subpopulations from control and Tip60^Δ/Δ E14.5 embryos from the same littermates: LSK (Lin⁻c-Kit⁺Sca-1⁺), LK (Lin⁻c-Kit⁺Sca-1⁻), LT-HSC (CD34⁻Flk2⁻LSK), ST-HSC (CD34⁺Flk2⁻LSK), MPP (CD34⁺Flk2⁺LSK), GMP (CD34⁺CD16/32⁺LK), CMP (CD34⁺CD16/32⁻LK), and MEP (CD34⁻CD16/32⁻LK). (F) Whole fetal liver cells (1 × 10⁶) from Tip60^f/+, Tip60^+/Δ, and Tip60^Δ/Δ E14.5 embryos were transplanted into lethally irradiated congenic mice (CD45.1⁺), along with 1 × 10⁵ congenic WBM (CD45.1⁺CD45.2⁺) cells. Donor chimerism of recipient PB is shown. (G) Donor chimerism of recipient BM HSCs (CD34⁺ Flk2⁻LSK) at 16 weeks after the transplantation. The values are presented as means ± standard error of the mean. Statistical analyses were performed vs Tip60^f/+. *P < .05; **P < .01; ***P < .001, ns; not significant.