Disabling p53 rescues clonogenesis and slows differentiation in FANCA-deficient cells. (A-B) The indicated human IPSC lines were exposed to 1-Gy ionizing radiation, and expression of TP53 and CDKN1A was analyzed 4 hours later by quantitative PCR. Results are aggregated from 2 independent experiments and analyzed by a Student t test. (C-D) FANCA-deficient HPCs generated in the absence of doxycycline with or without TP53 disruption were stained for Ki67 and DAPI to analyze cell cycle status. Results are aggregated from 3 parental IPSC lines and compiled over 2 independent experiments and analyzed by a Student t test. (E) The indicated HPCs isolated at day 8 of EHT were plated in methylcellulose in the presence of hematopoietic cytokines (10 000 cells/assay), and 14 days later colonies were scored (n = 4 biologic replicates over 3 independent experiments, and total colony numbers were compared by a Student t test, results are presented as mean ± SEM for each aggregated colony type). (F-G) The indicated cell lines were cultured under pro-erythroid differentiation conditions and analyzed by flow cytometry at the indicated time points. CD49d/Band 3 plots are from the GLYA⁺ population of the differentiating culture. Results are aggregated over 3 independent experiments and compared by a Student t test.