Mitochondrial respiration analysis in T-cell subsets. T cells from normal donors were sorted into 3 subsets and exposed to chemotherapy for 24 hours, allowed to recover, and then either left unstimulated or exposed to CD3/28 beads for 48 hours. Parameters assessed by row are OCR (A), ECAR (B), SRC (C), and glucose uptake (D). Doxorubicin at 400 nM reduces all aspects of respiration in unstimulated naive T cells with a corresponding increase in glucose uptake. Cyclophosphamide and cytarabine (Cytara) are detrimental to nearly all subsets. Stimulation largely rescues the doxorubicin effects but reveals deficits in cyclophosphamide- and cytarabine-exposed T cells. Glucose uptake after stimulation is only affected by cytarabine in naive and CM T cells. *P < .01.