Mitochondrial respiration analysis of T cells exposed to chemotherapy (long-term stimulation). In this set of assays, T cells are exposed to chemotherapy for 24 hours, and then allowed to recover and left unstimulated or exposed to CD3/28 beads for 7 days followed by a rest down period of ∼6 days mimicking CAR manufacture. Parameters assessed by row are OCR (A), ECAR (B), SRC (C), basal glycolytic rate (D), and compensatory glycolytic rate (E). Under unstimulated conditions, any amount of chemotherapy is devastating to mitochondrial respiration and glycolytic energy production. With stimulation, surviving T cells have variable alterations characterized most notably by an increase in ECAR and glycolysis in doxorubicin- and cytarabine-exposed T cells and a loss of SRC in cyclophosphamide-exposed T cells. *P < .05.