DRP reduces incidence of vaso-occlusion in the liver of transgenic SCD mice. (A-B) Still images obtained using qLIM techniques. Texas Red–dextran and Ly6G green were used to stain the blood plasma and neutrophils, respectively. RBCs can be discerned as dark shapes within the vessels and were not stained. Microscopy was performed using a Nikon MPE multiphoton excitation microscope in collaboration with the Center for Biologic Imaging (CBI), University of Pittsburgh. Yellow outlines denote areas identified as vessels. *Regions of vaso-occlusion where loss of blood flow was detected. Scale bars represent 20 µm. Three SCD mice treated with LPS (39 FOVs) and 3 SCD mice treated with both LPS and DRPs (30 FOVs) were used in this study. Blood flow in ∼30 blood vessels was assessed in each FOV. Quantification of the percentage of total flowing vessel area per FOV (C), and quantification of the percentage of total flowing vessels per FOV (D) by their individual count. (E) Number of neutrophil-containing vaso-occlusions per FOV in SCD mice. Data presented as mean ± standard error; ***P < .001.