Experimental setup and functional validation of LSC populations. (A) Integration of proteomic (top row) and transcriptomic (bottom row) data sets of healthy HSPC (left column), LSC and non-LSC populations (right column). Proteome of AML samples (top right) was published earlier.¹²  (B) Leukemic engraftment potential of sorted populations per patient. Mustard shading indicates populations used for proteomics and transcriptomics; light mustard shading, populations used for additional transcriptomics. (C) Flow cytometric analysis of CD34 and CD38 expression of pooled healthy samples used for proteome. Live, lineage-negative cells are shown, and numbers represent gated events of phenotypic HSPCs. NK, normal karyotype.