Carbon nucleophiles BTD and CHD inhibit oxLDL/CD36–induced PSer externalization. (A) Dot plots of annexin V binding by DMSO or 2 mM BTD treatment in oxLDL stimulation alone or oxLDL pretreatment with CVX stimulation. (B-C) Percent positive annexin V binding in the time course stimulation with oxLDL alone or oxLDL with CVX in BTD- (B) and CHD-treated (C) samples. Washed human platelets (30 ×10³/µL) were pretreated with 2 mM BTD or 5 mM CHD for 15 minutes at 37°C. After the addition of 1 mM CaCl₂/MgCl₂, platelets were stimulated with 50 µg/mL oxLDL for up to 30 minutes. In some conditions, platelets were subjected to 5 minutes of costimulation with 500 ng/mL CVX following the time course treatment with oxLDL. P values were determined by 1-way analysis of variance with 2-stage Benjamin, Krieger, and Yekutieli post hoc analysis. *P < .05 compared with DMSO, no stimulation (no oxLDL, no CVX); #P < .05 compared with DMSO, CVX alone (no oxLDL); ##P < .01 compared with DMSO, CVX alone (no oxLDL); †P < .05 compared with their respective DMSO control; and ††P < .01 compared with their respective DMSO control. N = 6 separate donors for the BTD treatments. N = 3 separate donors for the CHD treatments. Data are expressed as mean ± SEM.