Figure 1.
S-2HG treatment increases the CCR7+/CD45RO+(TCM) population in Pan T cells. (A) Flow cytometry plots of CD8 T cells showing surface expression of CCR7 and CD45RO following activation of pan T cells cultured with either IL-2 (30 U/mL) or IL-7/IL-15 (10 ng/mL) or IL-15 (10 ng/mL). Cells were treated with vehicle (H2O) or S-2HG (0.6 mM) from days 0 to 8 and analyzed at day 8 by flow cytometry. Representative plots from 3 individual donors are shown. (B) Quantification of panel A. The proportion of CD8 TCM (CCR7+/CD45RO+) cells for the different conditions is shown (mean ± standard error of the mean [SEM]). Two-way analysis of variance (ANOVA) with the Sidak multiple comparison test comparing the means of S-2HG to the means of vehicle for each culturing condition. (C) Flow cytometry plots of CD4 T cells showing surface expression of CCR7 and CD45RO following activation of pan T cells cultured with either IL-2 (30 U/mL) or IL-7/IL-15 (10 ng/mL) or IL-15 (10 ng/mL). Cells were treated with vehicle (H2O) or S-2HG (0.6 mM) from days 0 to 8 and analyzed at day 8 by flow cytometry. Representative plots from 3 individual donors are shown. (D) Quantification of panel C. The proportion of CD4 TCM (CCR7+/CD45RO+) cells for the different conditions is shown (mean ± SEM). Two-way ANOVA with the Sidak multiple comparison test comparing the means of S-2HG to the means of vehicle for each culturing condition. For all panels: **P ≤ .01; ***P ≤ .001; ****P ≤ .0001.

S-2HG treatment increases the CCR7+/CD45RO+(TCM) population in Pan T cells. (A) Flow cytometry plots of CD8 T cells showing surface expression of CCR7 and CD45RO following activation of pan T cells cultured with either IL-2 (30 U/mL) or IL-7/IL-15 (10 ng/mL) or IL-15 (10 ng/mL). Cells were treated with vehicle (H2O) or S-2HG (0.6 mM) from days 0 to 8 and analyzed at day 8 by flow cytometry. Representative plots from 3 individual donors are shown. (B) Quantification of panel A. The proportion of CD8 TCM (CCR7+/CD45RO+) cells for the different conditions is shown (mean ± standard error of the mean [SEM]). Two-way analysis of variance (ANOVA) with the Sidak multiple comparison test comparing the means of S-2HG to the means of vehicle for each culturing condition. (C) Flow cytometry plots of CD4 T cells showing surface expression of CCR7 and CD45RO following activation of pan T cells cultured with either IL-2 (30 U/mL) or IL-7/IL-15 (10 ng/mL) or IL-15 (10 ng/mL). Cells were treated with vehicle (H2O) or S-2HG (0.6 mM) from days 0 to 8 and analyzed at day 8 by flow cytometry. Representative plots from 3 individual donors are shown. (D) Quantification of panel C. The proportion of CD4 TCM (CCR7+/CD45RO+) cells for the different conditions is shown (mean ± SEM). Two-way ANOVA with the Sidak multiple comparison test comparing the means of S-2HG to the means of vehicle for each culturing condition. For all panels: **P ≤ .01; ***P ≤ .001; ****P ≤ .0001.

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