TDZ-induced effects. In t(6;11)AML cells, where AF6 is sequestered in the nucleus, TDZ binds S100A8-A9-ANXA6, impairing actin turnover, promoting F-actin aggregates, and ultimately leading to massive Ca²⁺ influx, which in turn promotes ROS overproduction and mitochondrial depolarization, triggering cell death. Conversely, in non-t(6;11)AML cells, TDZ still binds S100A8-A9-ANXA6, but the functional cytoplasmic AF6 counteracts its action, inducing milder cytoskeletal rearrangement and Ca²⁺ influx, eliciting reversible effects.