IgE-mediated PSA, PCA, and mast cell degranulation are attenuated in immunized NFATC2-deficient mice. (A) IgE-induced passive systemic anaphylaxis (PSA) is attenuated in NFATC2 KO mice. (B-C) Similarly, anti-DNP IgE-mediated passive cutaneous anaphylaxis (PCA) is attenuated in KO mice (B) and leads to fewer mast cells in the ear pinnae of mice (C). (D) IgE- but not IC-mediated ex vivo mast cell degranulation is attenuated in KO mice. (E) FcεRI expression on peritoneal mast cells (PMCs) is decreased by NATC2 deficiency. (F) FcγRIIB/RIII expression of PMCs is similar in WT and KO mice. (G) PMC FcεRI expression is induced by IgE to a similar extent in WT and KO mice. **P < .01; ***P < .001.