NFATC2 deficiency attenuates Th2 responses, decreases ASNase-specific CD4⁺T-cell proliferation, and increases regulatory T cells after ASNase immunization. (A-H) Naïve NFATC2 KO mice have more CD4⁺ INF-γ⁺ T cells (A) and higher plasma INF-γ levels (B) than WT mice. Naïve and immunized KO mice have fewer CD4⁺IL-4⁺ T cells (C) and less plasma IL-4 and IL-13 (D-E) compared with WT controls. After ASNase immunization, KO mice develop more CD4⁺ Tregs (F) and have higher plasma IL-10 (G) and TGF-β1 levels (H) than WT controls. (I) Immunized KO mice have decreased splenic CD4⁺ T-cell proliferation in response to ASNase or an ASNase T-cell epitope relative to immunized WT control mice. *P < .05; **P < .01; ***P < .001; ****P < .0001.