A total of 116 reported pathogenic variants available for manual review in paired samples from 34 patients. (A) Overview of reasons for variants not having been called in the paired sample and resulting changes in numbers of unique and shared variants. Six variants from 5 patients where presence could not be verified in the other sample because of lack of coverage, custom FLT3-ITD caller, and irretrievable BAM files are excluded. (B) VAFs of reported unique variants, grouped by lymphoid vs myeloid/ MPAL diagnoses and pathways. (C) VAFs of truly unique variants. All variants and VAFs were confirmed by manual review of BAM files.