Aldh3a2 is dispensable for normal hematopoiesis. (A) Aldh3a2 knockdown with Aldh3a2-sh-1 shows no difference in the number of N-GMPs in methylcellulose compared with N-GMPs infected with control shRNA (n = 3 wells per group). (B) Aldh3a2^−/− mice (KO) show one half of total aldehyde dehydrogenase enzyme activity in whole BM compared with Aldh3a2^+/+ mice (WT). (C-E) BM analysis showing frequency of Lin⁻cKit⁺Sca1⁺CD48⁻CD150⁺ hematopoietic stem cells (HSCs) (C), committed myeloid progenitors (common myeloid progenitors [CMPs], granulocyte-monocyte progenitors [GMPs], and megakaryocyte-erythrocyte progenitors [MEPs]) (D), and B cells (B220⁺) and myeloid cells (Mac1⁺) (E) in Aldh3a2-WT and Aldh3a2-KO mice. (F-G) Relative peripheral blood reconstitution (F) and contribution to B cells (B220⁺), myeloid cells (Mac1⁺), and T cells (CD3⁺) (G) 20 weeks after transplantation of recipient B6.SJL (CD45.1) mice transfused with whole BM cells from Aldh3a2-WT or KO mice (CD45.2⁺) competed with equal numbers of WT CD45.1 whole BM cells (n = 10 recipients per group). Data are representative of ≥2 independent experiments; n = 3 mice per genotype per experiment. Data are represented as mean ± standard deviation. P > .05 was considered nonsignificant (ns). ***P < .001.