Cluster analysis using the Hellinger distance generally recapitulates transplant recipient grouping. (A) Comparison of 3 recipient transplant and donor T-cell graft groups. The GI tract repertoire were compared between and across recipient groups restricted to syngeneic (C57BL/6), minor mismatch (BALB.B), and major mismatch (BALB/c) to obtain Hellinger distances that were then used to create a cluster (method) using the donor graft repertoire as an outgroup for TCRA (upper panel) and TCRB (lower panel). Results are from 1 representative experiment. (B) Comparison of 4 recipient and the donor GI tract groups. The Hellinger distance was obtained compared across each individual mouse in each individual group and used to generate an unbiased cluster. In both analyses, the syngeneic GI repertoire more closely associates with donor TCR.