Figure 4.
Tln1-mR35E,R118E platelets exhibit impaired αIIbβ3 activation to a similar extent as Rap1a/b-mKO platelets. (A-B) Flow cytometry assay to measure binding of GPIX-labeled platelets in whole blood to JonA/PE antibody in response to PAR4-AP (A) or convulxin (B) stimulation. Bar graphs represent mean fluorescence intensity (MFI) ± standard error of the mean (n = 6 mice, representative of ≥3 independent experiments). (C) Representative aggregation responses of Tln1-mR35E,R118E and Rap1a/b-mKO platelets stimulated with various concentrations of agonists. Curves corresponding to control and Tln1-mR35E,R118E platelets stimulated with PAR4-AP were from the same experiment as those depicted in Figure 3D. Arrows indicate addition of agonists. *P < .05;**P < .01; ***P < .001. ns, not significant.

Tln1-mR35E,R118E platelets exhibit impaired αIIbβ3 activation to a similar extent as Rap1a/b-mKO platelets. (A-B) Flow cytometry assay to measure binding of GPIX-labeled platelets in whole blood to JonA/PE antibody in response to PAR4-AP (A) or convulxin (B) stimulation. Bar graphs represent mean fluorescence intensity (MFI) ± standard error of the mean (n = 6 mice, representative of ≥3 independent experiments). (C) Representative aggregation responses of Tln1-mR35E,R118E and Rap1a/b-mKO platelets stimulated with various concentrations of agonists. Curves corresponding to control and Tln1-mR35E,R118E platelets stimulated with PAR4-AP were from the same experiment as those depicted in Figure 3D. Arrows indicate addition of agonists. *P < .05;**P < .01; ***P < .001. ns, not significant.

Close Modal
This Feature Is Available To Subscribers Only

or Create an Account

Close Modal
Close Modal